|
| Status |
Public on Apr 17, 2024 |
| Title |
WT D60 H3K4me3 Replicate 1 |
| Sample type |
SRA |
| |
|
| Source name |
matured cortical interneurons
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: matured cortical interneurons
stem cell_source: H9 hESC
passage: p10-p60
timepoint: D60
antibody: H3K4me3
|
| Growth protocol |
Embryonic stem cells were mainatined in mTeSR media on Vitronectin coated cultureware.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was processed using CUT&Tag (Epicypher) and isolated using phenol-chloroform follow by the NucleoSpin® Gel and PCR Clean-Up kit (Macherey-Nagel).
After PCR amplification samples with unique dual-end indexes were pooled in equimolar concentrations.
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Raw reads were processed using AIAP (v1.1) for trimming, alignment, bigWig generation and peak calling
Assembly: hg38
Supplementary files format and content: bigWig density file
Supplementary files format and content: narrowPeak or broadPeak file containing output from MACS2 peakcalling
Library strategy: CUT&Tag
|
| |
|
| Submission date |
Jul 27, 2023 |
| Last update date |
Apr 17, 2024 |
| Contact name |
Kristen Kroll |
| Organization name |
Washington University School of Medicine
|
| Department |
Developmental Biology
|
| Lab |
Kristen Kroll
|
| Street address |
320 McDonnell Sciences/660 S. Euclid Ave.
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE239400 |
Assessment of chromatin state changes during human cortical interneuron development |
| GSE239481 |
Defining Cis-regulatory Elements and Transcription Factors that Control Human Cortical Interneuron Development |
|
| Relations |
| BioSample |
SAMN36728803 |
| SRA |
SRX21172240 |
