|
| Status |
Public on Apr 26, 2012 |
| Title |
AB32_pCDH_vectorControl_6hORG1 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-10A clone AB32 cells grown in monolayer culture, transduced 48h with pCDH empty vector virus then treated 6h with 10nM ORG2058
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: breast
cell line: AB32
transduced virus: pCDH-CMV-mcs-EF1-copGFP
|
| Treatment protocol |
Cells were transduced with empty vector (pCDH) or FOXA1 expression viral particles 48h, then treated with 10nM ORG2058 or ethanol vehicle (0.1% final in growth medium) for the indicated times, followed by harvesting by trypsinization and centrifugation.
|
| Growth protocol |
Cells were grown in standard recommended media in monolayer cultures at 37C and 5% CO2 for 24h prior to viral transduction and treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Invitrogen RNaqueous reagents and following the standard protocol
|
| Label |
biotin
|
| Label protocol |
500ng total RNA per sample was amplified and biotin labeled using Invitrogen Illumina total Prep reagents, following the standard protocol
|
| |
|
| Hybridization protocol |
750ng biotin-labeled sample was hybridized to each HT-12 array using Illumina reagents and standard procedure
|
| Scan protocol |
The standard Illumina scanning protocol was used
|
| Description |
Replicate 1
|
| Data processing |
The data were background subtracted and normalized using the cubic spline normalization method using Illumina Genome Studio software
|
| |
|
| Submission date |
Aug 02, 2011 |
| Last update date |
Apr 26, 2012 |
| Contact name |
J Dinny Graham |
| E-mail(s) |
dinny.graham@sydney.edu.au
|
| Phone |
61 2 86273702
|
| Organization name |
University of Sydney
|
| Department |
Westmead Institute for Medical Research
|
| Lab |
Translational Breast Cancer Genomics
|
| Street address |
176 Hawkesbury Rd
|
| City |
Westmead |
| State/province |
NSW |
| ZIP/Postal code |
2145 |
| Country |
Australia |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE31127 |
Impact of FOXA1 over-expression on progestin signalling in transformed normal breast cells |
| GSE31130 |
Non-overlapping progesterone receptor cistromes contribute to cell-specific transcriptional outcomes in breast cells |
|
