|
| Status |
Public on Mar 20, 2024 |
| Title |
Muscle_+3h_trained_ET8 |
| Sample type |
RNA |
| |
|
| Source name |
vastus lateralis muscle, exercised, 60 min
|
| Organism |
Homo sapiens |
| Characteristics |
training status: trained
time point: +3h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
All muscle biopsies were taken under sterile conditions from the lateral portion of the m. vastus lateralis of the right leg using a fine-needle punch biopsy technique under local anesthesia (1% Meaverin). Snap-frozen muscle biopsy samples were homogenized with a TissueRuptor homogenizer (Qiagen, Hilden, Germany), and total RNA was extracted and purified using RNeasy Fibrous Tissue Mini kit and RNeasy Micro Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Affymetrix's SensationPlusTM FFPE Amplification and 3'IVT Labeling kit.
|
| |
|
| Hybridization protocol |
GeneAtlas Hybridization, Wash, and Stain Kit for 3' IVT Arrays, Affymetrix
|
| Scan protocol |
Scanned images (DAT files) were transformed into intensities (CEL files) by GCOS (Affymetrix).
|
| Description |
Gene expression data from vastus lateralis muscle 3 hours min after acute exercise.
All study participants absolved an acute exercise protocol that consisted of 60 min of high intensity cycling exercise on a cycle ergometer at a power requiring 80% of the individual VO2max (including 10 min of initial warm-up cycling at 60% VO2max). All exercise sessions were conducted in the morning (9:00 AM) after an overnight fast and after receiving a standardized breakfast (8:00 AM).
|
| Data processing |
Raw intensity values were background corrected, Log2 transformed, quartile normalized and summarized using the RMA algorithm in Genomics Suite 6.6 (Partek).
|
| |
|
| Submission date |
Dec 13, 2023 |
| Last update date |
Mar 20, 2024 |
| Contact name |
Thomas Beiter |
| E-mail(s) |
thomas.beiter@med.uni-tuebingen.de
|
| Phone |
4970712985233
|
| Organization name |
Eberhard-Karls-University Tübingen
|
| Department |
Sports Medicine
|
| Street address |
Hoppe-Seyler-Str. 6
|
| City |
Tübingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE250122 |
The impact of endurance exercise on human skeletal muscle transcriptome |
|
