|
| Status |
Public on Sep 16, 2011 |
| Title |
Macrophages where c-Myc activation was blocked by using c-Myc chemical inibitors, rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Human macrophages where c-Myc activation was blocked by using c-Myc chemical inibitors
|
| Organism |
Homo sapiens |
| Characteristics |
source tissue: normal donor buffy coats
source cell type: freshly isolated monocytes
cell type: macrophages
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 2.7 ug of cDNA were hybridized for 16 hr at 45C on Human Genome HuGene 1.0 ST . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Donor_3 MycI_rep3
R743_N9
|
| Data processing |
Expression dataset was obtained by means of Partek software version 6.3, with RMA method.
|
| |
|
| Submission date |
Sep 15, 2011 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Massimo Locati |
| E-mail(s) |
transcriptional.profiles@humanitasresearch.it
|
| Phone |
+39 02 8224 5116
|
| Fax |
02-82245101
|
| URL |
http://www.humanitas.it/hur/cms/english/activities/lab/leukocyte_biology/groupleader.html
|
| Organization name |
Istituto Clinico Humanitas
|
| Department |
Department of Translational Medicine of the University of Milan Faculty of Medicine, IRCCS Istituto Clinico Humanitas
|
| Lab |
Laboratory of Leukocyte Biology
|
| Street address |
Via Manzoni, 113
|
| City |
Rozzano |
| State/province |
MILAN |
| ZIP/Postal code |
20089 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE32164 |
Expression data from macrophage maturation and polarization c-Myc in alternative macrophage activation experiments |
|
| Relations |
| Reanalyzed by |
GSE86357 |
