|
| Status |
Public on Sep 26, 2012 |
| Title |
5019 SC GN |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral Blood Granulocytes
|
| Organism |
Homo sapiens |
| Characteristics |
disease: essential thrombocythemia
treatment: NO
|
| Treatment protocol |
Patients with polycythemia vera and essential thrombocythemia received the JAK1 and JAK2 inhibitor INCB018424; garnulocytes were isolated from peripheral blood and assayed for array. No further in vitro treatment was performed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA, including small RNAs, was extracted using RNeasy Micro kit (Qiagen, Valencia, CA) following the protocol supplied by the manufacturer. Disposable RNA chips (Agilent RNA 6000 Nano LabChip kit, Agilent Technologies, Waldbrunn, Germany) were used to determine the purity/integrity of RNA samples using Agilent 2100 Bioanalyzer. NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) was used to evaluate the RNA sample concentration, and 260/280 and 260/230 nm ratios were considered to verify the purity of total RNA.
|
| Label |
Biotin
|
| Label protocol |
Total RNA (500ng for samples) was labeled using the FlashTag® Biotin HSR kit (Genisphere)
|
| |
|
| Hybridization protocol |
Total labeled RNA was hybridized to the Affymetrix Genechip miRNA array 1.0 using manufacturer's protocols.
|
| Scan protocol |
Image files from scanning (.dat and .cel files) were generated with Affymetrix Expression Console package.
|
| Description |
miRNA expression profiles of in granulocytes in patients with polycythemia vera (PV) and essential thrombocythemia (ET) receiving INCB018424
|
| Data processing |
Partek GS was then used to generate signals using RMA procedure.
|
| |
|
| Submission date |
Sep 16, 2011 |
| Last update date |
Sep 26, 2012 |
| Contact name |
Enrico Tagliafico |
| E-mail(s) |
enrico.tagliafico@unimore.it
|
| Organization name |
University of Modena and Reggio Emilia
|
| Department |
Center for Genome Research
|
| Street address |
Via Campi 287
|
| City |
Modena |
| State/province |
MO |
| ZIP/Postal code |
41126 |
| Country |
Italy |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE32194 |
Changes of miRNA expression in granulocytes of patients with polycythemia vera (PV) and essential thrombocythemia (ET) receiving INCB018424 in a Phase 2 study |
|
