|
| Status |
Public on Dec 01, 2012 |
| Title |
newborn (P0) Krt1+/+ skin_replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse full-thickness skin
|
| Organism |
Mus musculus |
| Characteristics |
tissue: full-thickness skin
age: newborn (P0)
genotype/variation: Krt1+/+
strain: C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalised using quantile normalisation in R
|
| |
|
| Submission date |
Oct 13, 2011 |
| Last update date |
Jan 23, 2013 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
j.schultze@uni-bonn.de
|
| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE32951 |
Genome-wide analysis of gene expression in Krt1+/+ versus Krt1-/- epidermis and full-thickness skin. |
|
