|
| Status |
Public on Aug 01, 2012 |
| Title |
Nalm6_MondoA_knockdown_3 |
| Sample type |
RNA |
| |
|
| Source name |
MondoA shRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Nalm6
cell type: leukemia
|
| Growth protocol |
Cells were grown in plastic cell culture flasks in a humidified incubator at 37°C in 5% CO2 atmosphere in RPMI 1640 media containing 20% heat-inactivated fetal bovine serum, 1% glutamine, 100 µg/mL gentamycin and 200 mg/dl D-glucose .
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNeasy RNA isolation kit according to the manufacturer´s instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA (200 ng) was amplified and labeled using Affymetrix GeneChip Whole Transcript Sense Target Labeling Kit.
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Hybridization, Wash, and Stain Kit. cRNA was hybridized to Affymetrix Human Gene 1.0 ST arrays
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
The data were analyzed with Affymetrix Expression Console 1.1 using Affymetrix default analysis settings and RMA as normalization method.
|
| |
|
| Submission date |
Nov 28, 2011 |
| Last update date |
Aug 01, 2012 |
| Contact name |
Olivia Prazeres da Costa |
| E-mail(s) |
olivia.p.dacosta@googlemail.com
|
| Phone |
00498941404149
|
| Organization name |
Technische Universität München
|
| Department |
Medical Microbiology, Immunology and Hygiene
|
| Street address |
Trogerstr. 30
|
| City |
München |
| State/province |
Bavaria |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE33967 |
MondoA is highly expressed in acute lymphoblastic leukemia and modulates metabolism, differentiation and survival |
|
