|
| Status |
Public on May 29, 2013 |
| Title |
Human neural progenitor cells, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
85 NPC miRNA 4-23-10_(miRNA-1_0_2Xgain).CEL
|
| Organism |
Homo sapiens |
| Characteristics |
source tissue: Fetal brain source tissue
gestation: 84 days in utero
cell type: neural progenitor cells
|
| Treatment protocol |
Dissociated fetal tissue is placed in neural progentior media for 1 week to give rise to neurospheres or neural progenitor cells (NPCs).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
NPCs were placed in differentiation media for 2-3weeks to differentiate into neurons.
|
| Label |
FlashTag Biotin HSR labeling was performed according to the Genisphere protocol from 200ng total RNA (Genisphere Technical Support).
|
| Label protocol |
FlashTag Biotin HSR labeling was performed according to the Genisphere protocol from 200ng total RNA (Genisphere Technical Support).
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the affymetrix protocol. GeneChips were washed and stained in the Affymetrix Gene chip system.
|
| Scan protocol |
miRNA GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
miRNA expression data from neural progenitor cells
|
| Data processing |
The data were analyzed using the Partek Genomics Suite and probe sets mapped to genes using the database for annotation.
|
| |
|
| Submission date |
Nov 29, 2011 |
| Last update date |
May 29, 2013 |
| Contact name |
Sowmya V Yelamanchili |
| E-mail(s) |
syelamanchili@unmc.edu
|
| Phone |
402-559-5348
|
| Organization name |
University of Nebraska Medical center
|
| Department |
Pharmacology and Experimental Neuroscience
|
| Street address |
985800 Nebraska Medical Center - DRC 1 3008
|
| City |
Omaha, |
| State/province |
NE |
| ZIP/Postal code |
68198-5800 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE34016 |
MicroRNAs in neuronal differentiation |
|
