|
| Status |
Public on Sep 04, 2012 |
| Title |
WB_C0005 |
| Sample type |
RNA |
| |
|
| Source name |
Whole blood, symptomatic
|
| Organism |
Homo sapiens |
| Characteristics |
sample name: PAW152
genotypic pc1: -0.0741
genotypic pc2: 0.0379
genotypic pc3: -0.0414
location: Cotonou
gender: F
age (years): 2
malaria rd test: POSITIF
parasitemia: 20000
log2 parasitemia: 14.29
parasitemia category: High Parasitemia
red blood cells: 4.2
white blood cells: 10
hemoglobin genotype: AA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from whole blood collected in Tempus tubes using the Tempus Spin RNA Isolation kit (Applied Biosystems). Total RNA was then globin-mRNA-depleted using GlobinClear kit (Ambion). Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
globin mRNA-depleted total RNA
|
| Data processing |
The data were normalised using quantile normalisation with JMP Genomics
|
| |
|
| Submission date |
Dec 13, 2011 |
| Last update date |
Nov 14, 2012 |
| Contact name |
Youssef Idaghdour |
| E-mail(s) |
youcarolina@gmail.com
|
| Organization name |
NCSU
|
| Department |
Genetics
|
| Lab |
Gibson
|
| Street address |
Gardner Hall NCSU Campus
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27695 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE34404 |
The genomic architecture of host whole blood transcriptional response to malaria infection |
|
