|
| Status |
Public on Jan 01, 2012 |
| Title |
T5KO + PBS 3 |
| Sample type |
RNA |
| |
|
| Source name |
T5KO + PBS
|
| Organism |
Mus musculus |
| Characteristics |
mouse ko type: T5KO
tissue type: Colon
treatment: PBS
|
| Treatment protocol |
Flagellin (FliC) from WT Salmonella enterica serovar Typhimurium (SL3201, fljB–) was purified through sequential cation and anion-exchange chromatography and purity was verified as previously described.
|
| Growth protocol |
Generation of WT, T5KO, N4KO and T5/N4-DKO was previously described 7. Mice were bred and maintained at Emory University and used between 6 and 8 weeks of age.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy kits (QIAGEN, Valencia, CA) according to manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
PCR product was prepared with standard Illumina protocol for arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
BeadChips were scanned using Illumina BeadArray Scanner
|
| Data processing |
The data were normalized using quantile-normalization with GenomeStudio V2010.2
|
| |
|
| Submission date |
Dec 16, 2011 |
| Last update date |
Jan 01, 2012 |
| Contact name |
Gregory Doho |
| E-mail(s) |
gdoho@emory.edu
|
| Organization name |
Emory University
|
| Street address |
1365B Clifton Rd NE
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE34492 |
Cytosolic flagellin receptor NLRC4 protects mice against mucosal and systemic challenges |
|
