|
| Status |
Public on Apr 01, 2012 |
| Title |
Sca1+ muscle interstitial cells isolated from TSA treated MDX mice (45 days) replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Sca1+ muscle interstitial cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: DMD/MDX
tissue: Muscle
cell type: interstitial cell (CD34+/a7-integrin+/Sca1+)
agent: TSA
|
| Treatment protocol |
Mice were treated with TSA (0.6 mg/Kg) or veichle for 45 days (i.p. injection)
|
| Growth protocol |
Cells where isolated from DMD/MDX mice using FACS (Fluorescence Activade Cell Sort); Sca1+ muscle interstitial cells were isolated as CD34+/a7-integrin+/Sca1+ while MuSC were isolated as CD34+/a7-integrin+/Sca1-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was purified with Rneasy kit (Qiagen) following the manifacturers protocol
|
| Label |
streptavidin Cy3
|
| Label protocol |
As suggested by Agilent
|
| |
|
| Hybridization protocol |
As recommended by Agilent
|
| Scan protocol |
As suggested by Agilent using the illumina scanner
|
| Description |
Raw data file:
US83203534_252800510534_S01_GE1_107_Sep09_2_1.txt
gene expression of Sca1+ muscle interstitial cells isolated from TSA treated MDX mice (45 days) replicate 1
|
| Data processing |
Raw data filtered on the absent / detected flag calculated by the Agilent algorithum in GeneSpring GX11 (The data was normalized with quantiles).
|
| |
|
| Submission date |
Jan 04, 2012 |
| Last update date |
Apr 01, 2012 |
| Contact name |
Roy M Williams |
| E-mail(s) |
rwillia@scripps.edu
|
| Organization name |
The Scripps Research Institute
|
| Department |
Regenerative Medicine
|
| Lab |
Loring
|
| Street address |
3030 Science Park Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE34843 |
HDAC inhibitors promote the pro-myogenic activity of Sca1-positive interstitial cells in regenerating dystrophic muscles |
|
