|
| Status |
Public on Mar 30, 2012 |
| Title |
CD11b+L6G-Ly6C+ cells, 6wks tumor-bearing mice, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
CD11b+L6G-Ly6C+ cells from 6wks tumor-bearing mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: BALB/c
gender: female
tissue: spleen
cell type: CD11b+L6G-Ly6C+ Mo-MDSCs
tumor injection: 1x10^5 Her-2/CT26 cells
days after tumor injection: 6 weeks
|
| Treatment protocol |
To obtain MDSC subpopulations, CD11b+ cells, enriched by the MACS system, were stained with APC-conjugated anti-CD11b Ab, FITC-conjugated anti-Ly-6C Ab and PE-conjugated anti-Ly-6G Ab and sorted using a FACSAria II (BD Bioscience).
|
| Growth protocol |
BALB/c mice were subcutaneously injected with 1x10^5 Her-2/CT26 cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified from CD11b+Ly-6C(high)Ly-6G(low) and CD11b+Ly-6C(low)Ly-6G(high) cells using Qiagen RNeasy kits (Qiagen, Valencia, CA).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Wt_sensetarget_label_manual(updated manual)_1, 2007, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization cocktails containing 5 to 5.5 µg of fragmented, end-labeled single-stranded cDNA were prepared and hybridized to GeneChip Human Gene 1.0 ST arrays for 17hr at 45°C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
LR1-3
Gene expression data from MDSCs.
|
| Data processing |
The RMA algorithm was used for expression summary and signal calculation of Affymetrix MoGene 1.0 ST array data. Sketch Quantile normalization was performed for the signal normalization.
|
| |
|
| Submission date |
Jan 28, 2012 |
| Last update date |
Mar 30, 2012 |
| Contact name |
Chang-Yuil Kang |
| E-mail(s) |
cykang@snu.ac.kr
|
| Phone |
82-2-880-7860
|
| Organization name |
Seoul National University
|
| Street address |
29-102, Seoul National University, Shillim-dong, Gwanak-gu
|
| City |
Seoul |
| ZIP/Postal code |
151-742 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE35398 |
Expression data from MDSCs at different time points during tumor progression |
|
