|
| Status |
Public on Jul 12, 2012 |
| Title |
FH46 |
| Sample type |
RNA |
| |
|
| Source name |
right ventricle_fetal
|
| Organism |
Homo sapiens |
| Characteristics |
group: fetal heart samples
tissue: right ventricular myocardium
|
| Treatment protocol |
All tissue samples removed during surgery were excised by the performing surgeon for clinical indications utilizing standard of care procedures.
|
| Growth protocol |
Tissues samples obtained during patient surgery were immediately de-identified and frozen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from ~10 mg of frozen tissue from right ventricle using a mirVana microRNA isolation kit (Applied Biosystems/Ambion, Austin, TX) according to manufactures instruction
|
| Label |
FlashTag Biotin HSR
|
| Label protocol |
All arrays were run at the Kansas University Medical Center-Microarray Facility (KUMC-MF) according to the manufacturer’s protocols
|
| |
|
| Hybridization protocol |
All arrays were run at the Kansas University Medical Center-Microarray Facility (KUMC-MF) according to the manufacturer’s protocols
|
| Scan protocol |
All arrays were run at the Kansas University Medical Center-Microarray Facility (KUMC-MF) according to the manufacturer’s protocols
|
| Description |
FH46
|
| Data processing |
All statistical analyses were performed using statistical software: SPSS, Partek (Partek, Inc. St Louis, Mo.). Robust Multichip Analysis (RMA) was used for background correction followed by quantile normalization with baseline transformation to the median of the control samples
|
| |
|
| Submission date |
Feb 01, 2012 |
| Last update date |
Jul 12, 2012 |
| Contact name |
Douglas C Bittel |
| E-mail(s) |
dbittel@cmh.edu
|
| Organization name |
Childrens Mercy Hospital
|
| Department |
Genetics research
|
| Lab |
Developmental genomics
|
| Street address |
2401 Gillham Rd
|
| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64108 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (2) |
| GSE35490 |
Noncoding RNA expression in myocardium from infants with tetralogy of Fallot [miRNA profiling] |
| GSE35781 |
Noncoding RNA expression in myocardium from infants with tetralogy of Fallot |
|
