|
| Status |
Public on Dec 31, 2013 |
| Title |
heart Saline control rep6 |
| Sample type |
RNA |
| |
|
| Source name |
heart
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: left ventricle of heart
strain: Sprague-Dawley
treatment: saline control
|
| Treatment protocol |
Seventeen rats were randomized to receive 12 mg/kg doxorubicin or saline and sacrificed after 48 hours.
|
| Growth protocol |
Rats were subjected to 12 hour dark/light and ad libitum food and water
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from PBMCs and left heart ventricle using the AllPrep DNA/RNA/protein mini kit from Qiagen. RNA quality and quantity were measured on the Agilent 2100 bioanalyzer
|
| Label |
biotin-Cy3
|
| Label protocol |
RNA was amplified using the Nugen PicoSL WTA system and labeled with the Encore Biotin IL module kit prior to hybridization of samples to beadchips
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
left ventricle of heart was used
|
| Data processing |
The probe intensities were log2 transformed and averaged over samples. Within each sample the median of the log2-transformed intensities of the not expressed probes was subtracted from the log2 intensities to normalize the data
|
| |
|
| Submission date |
Apr 13, 2012 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Valentina Todorova |
| E-mail(s) |
vtodorova@uams.edu
|
| Organization name |
UAMS
|
| Street address |
4301 W. Markham
|
| City |
Little Rock |
| State/province |
AR |
| ZIP/Postal code |
72205 |
| Country |
USA |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE37260 |
Analysis of potential transcriptomic biomarkers for doxorubicin cardiotoxicity in peripheral blood |
|
