|
| Status |
Public on Jul 31, 2013 |
| Title |
ppabi1a/b 12hr 10 μM ABA |
| Sample type |
RNA |
| |
|
| Source name |
ppabi1a/b protonemata, 12 h 10 μM ABA
|
| Organism |
Physcomitrium patens |
| Characteristics |
tissue: protonemata
treatment: 10 μM ABA
time: 12h
genotype: ppabi1a/b
|
| Treatment protocol |
Wildtype and PpABI1 disruptants one-week-old protonemata were transferred to 1 μM or 10 μM ABA contain BCDAT media. The protonemal tissue were incubated for 1, 3, 6 and 12 hours at 25 ºC under continuous light.
|
| Growth protocol |
P. patens subspecies patens (Gransden) was the wildtype strain. Protonemal tissue was grown at 25 ºC under continuous light (50 - 80 µmol photons m-2 s-1) on 0.8% (w/v) agar plates of modified BCDAT medium [1.84 mM KH2PO4 (pH 6.5), 10 mM KNO3, 1 mM MgSO4, 1 mM CaCl2, 45 µM FeSO4, 10 µM H3BO3, 2 µM MnCl2, 0.22 µM CuSO4, 0.23 µM CoCl2, 0.19 µM ZnSO4, 0.1 µM Na2MoO4, 5 mM ammonium tartrate]
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted from ABA-treated or non-treated one-week-old protonemal tissues using RNeasy plant mini Kit (QIAGEN)
|
| Label |
Cy3
|
| Label protocol |
Labeling were performed by Agilent one-color method according to Agilent manufactures’ instruction
|
| |
|
| Hybridization protocol |
Hybridization were performed by Agilent one-color method according to Agilent manufactures’ instruction
|
| Scan protocol |
Scanning were performed by Agilent one-color method according to Agilent manufactures’ instruction
|
| Description |
Gene expression after 12 hours in 10uM ABA treated ppabi1a/b protonemata
|
| Data processing |
Spot signal values were calculated by Feature Extraction ver.9.1software supplied by Agilent
|
| |
|
| Submission date |
May 01, 2012 |
| Last update date |
Jul 31, 2013 |
| Contact name |
Kenji KOMATSU |
| E-mail(s) |
k4komats@nodai.ac.jp
|
| Phone |
+81-3-5477-2468
|
| Organization name |
Tokyo University of Agriculture
|
| Department |
Department of Bioproduction Technology
|
| Street address |
1-1-1 Sakuragaoka
|
| City |
Setagaya-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
156-8502 |
| Country |
Japan |
| |
|
| Platform ID |
GPL15514 |
| Series (1) |
| GSE37694 |
Genome-wide expression profiling of PpABI1 disruptants |
|
