|
| Status |
Public on May 05, 2012 |
| Title |
11178.s1 |
| Sample type |
RNA |
| |
|
| Source name |
LCLs_Control_2_male
|
| Organism |
Homo sapiens |
| Characteristics |
famid: 11178
simons simplex collection id: SSC00368
cell line: lymphoblast cell lines (LCLs)
cell type: lymphoblasts
chip id: X4801420043_G
project: 2009-084B
batch: 2
gender: male
age: 5.25
autism trait: Control
|
| Growth protocol |
The live lymphoblast cell lines (LCLs) of the subjects were grown in RPMI 1640 medium with 2 mM L-glutamine and 25 mM HEPES (Invitrogen, Carlsbad, CA, USA), 10% fetal bovine serum, and 1 × Antibiotic-Antimycotic solution (Invitrogen) at 37°C in a humidified 5% CO2 chamber. Cells were grown to a density of 6 × 10^5/ml.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
A total of 9 × 10^6 of lymphoblasts were seeded in a T-75 flask in 30 ml of fresh medium. After 24 h, total RNA was extracted from the cells using an RNeasy Mini Kit with DNase treatment (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Outlier samples were first removed followed by quantile normalisation and combat. All data processing steps were done in R (version 2.14.2).
|
| |
|
| Submission date |
May 04, 2012 |
| Last update date |
May 05, 2012 |
| Contact name |
Rui Luo |
| E-mail(s) |
luoruicd@gmail.com
|
| Organization name |
UCLA
|
| Department |
Human Genetics
|
| Lab |
Daniel Geschwind Lab
|
| Street address |
2309 Gonda Bldg, 695 Charles E. Young Dr. South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-1761 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE37772 |
Genome-wide transcriptome profiling reveals the functional impact of rare de novo and recurrent CNVs in autism spectrum disorders. |
|
