|
| Status |
Public on Feb 10, 2013 |
| Title |
mouse 495 left kidney ligature |
| Sample type |
RNA |
| |
|
| Source name |
mouse kidney ligatured
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD-1
tissue: ligatured left kidney
cell type: Primary cells
|
| Treatment protocol |
Kidney Ligature in vivo in mouse (mus musculus CD-1)
|
| Growth protocol |
Primary cells from mouse kidney ligatured or not
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Agilent Gene Expression Hybridization Kit (Agilent Technologies)
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled fragmented cRNA
|
| |
|
| Hybridization protocol |
hybridization overnight (17hours, 65°C), washing: Agilent gene expression wash buffer 1 for 1 min at room temperature followed by a second wash with preheated Agilent gene expression wash buffer 2 (37°C) for 1 min, last acetonitrile.
|
| Scan protocol |
Agilent's microarray scanner system (Agilent Technologies)
|
| Description |
SAMPLE 2
|
| Data processing |
The Agilent G2567AA Feature Extraction Software (v.9.1) was used to process the microarray files. The software determines feature intensities (including background subtraction), rejects outliers and calculates statistical confidences. Raw data were normalized by dividing the intensity values by their mediane
|
| |
|
| Submission date |
May 22, 2012 |
| Last update date |
Feb 10, 2013 |
| Contact name |
christophe desterke |
| E-mail(s) |
christophe.desterke@inserm.fr
|
| Phone |
+33 (0)145595316
|
| Organization name |
university Paris Sud11
|
| Department |
UFR medecine institute Andre Lwoff
|
| Lab |
hopital Paul Brousse Batiment Lavoisier
|
| Street address |
14-16 avenue Paul Vaillant Couturier
|
| City |
VILLEJUIF |
| ZIP/Postal code |
94807 |
| Country |
France |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE38117 |
Exploring new pathways involved during experimental renal fibrogenesis in the Unilateral Ureteral Obstruction |
|
