|
| Status |
Public on Aug 01, 2012 |
| Title |
5aza-dC |
| Sample type |
RNA |
| |
|
| Source name |
NIH/3T3
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Fibroblasts
cell line: NIH/3T3
treatment: 1uM 5aza-dC
|
| Treatment protocol |
1uM 5aza-dC was added and replaced daily for 3 days.
|
| Growth protocol |
Cells were maintained in Dulbecco's modified Eagle's medium supplemented with l-glutamine, non-essential amino acids, sodium pyruvate, β-mercapto-ethanol, and 15% foetal calf serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TRIZOL
|
| Label |
Biotin
|
| Label protocol |
TotalPrep RNA amplification kit
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Standard Illumina protocol
|
| Description |
1uM 5aza-dC
|
| Data processing |
Average Normalisation, Background subtracted
|
| |
|
| Submission date |
May 29, 2012 |
| Last update date |
Aug 01, 2012 |
| Contact name |
Jamie Hackett |
| E-mail(s) |
jah225@cam.ac.uk
|
| Organization name |
Gurdon Institue
|
| Street address |
Tennis Court Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 1QN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE38305 |
Promoter DNA methylation couples genome-defense mechanisms to epigenetic reprogramming in the mouse germline (part 1) |
| GSE38307 |
Promoter DNA methylation couples genome-defense mechanisms to epigenetic reprogramming in the mouse germline |
|
