|
| Status |
Public on Jan 01, 2013 |
| Title |
Primary tumor 14 |
| Sample type |
genomic |
| |
|
| Source name |
primary intrahepatic cholangiocarcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: primary intrahepatic cholangiocarcinoma
sample type: tumor tissue
|
| Growth protocol |
Cell lines were grown in RPMI-1640 supplemented with glutamine (2mM) and 10% fetal bovine serum in humidified 5% CO2 in air incubators at 37 ̊C.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted and purified from cell lines using the Qiagen DNeasy Tissue Kit according to standard instructions.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol.
|
| |
|
| Hybridization protocol |
Bisulphite-converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation27 Beadchips using the standard Illumina protocol.
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner settings.
|
| Description |
U059-T
Primary intrahepatic cholangiocarcinoma, Thailand.
|
| Data processing |
BeadStudio software methylation module 1.0.5.
|
| |
|
| Submission date |
Jun 21, 2012 |
| Last update date |
Jul 29, 2013 |
| Contact name |
Wei Dai |
| E-mail(s) |
w.dai@imperial.ac.uk
|
| Organization name |
Imperial College London
|
| Department |
Sugery and Cancer
|
| Lab |
Epigenetics
|
| Street address |
Du Cane Road
|
| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL8490 |
| Series (1) |
| GSE38860 |
Aberrant DNA methylation at genes associated with a stem cell-like phenotype in cholangiocarcinoma tumors |
|
