|
| Status |
Public on Dec 01, 2013 |
| Title |
HEK293 ALKBH7 uninduced replicate 3, Illumina HumanMethylation27 BeadChip |
| Sample type |
genomic |
| |
|
| Source name |
HEK293
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293
expression: ALKBH7 uninduced
cell type: Human Embryonic Kidney
provider: Life Technologies
catalog number: R750-07
|
| Growth protocol |
HEL293 were grown in Dulbecco’s modified Eagle’s medium (DMEM) (Lonza) supplemented with 10 % tetracycline-free fetal bovine serum (FBS) (Clontech), 100 U/ml penicillin (Lonza), 100 U/ml streptomycin (Lonza), 2 mM L-glutamine (Lonza), 15 μg/ml blasticidin-S (Invitrogen) and 200 μg/ml hygromycin B.), at 37°C with 5 % CO2. All cells were split when reaching 80 % confluency.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated using the QIAamp DNA mini kit from Qiagen.
|
| Label |
Biotin and dinitrophenyl
|
| Label protocol |
Standard Ilumina label protocol
|
| |
|
| Hybridization protocol |
Standard Ilumina hyb protocol
|
| Scan protocol |
Standard Ilumina scan protocol
|
| Data processing |
Data extraction and initial quality control of the bead summary raw data were performed using BeadStudio v3.1.0.0 and the Methylation module v1.9, both provided by Illumina.
|
| |
|
| Submission date |
Jul 05, 2012 |
| Last update date |
Dec 01, 2013 |
| Contact name |
Leonardo A. Meza-Zepeda |
| E-mail(s) |
leonardm@rr-research.no
|
| Organization name |
The Norwegian Radium Hospital
|
| Department |
Dept. of Tumor Biology
|
| Street address |
Ullernchausseen 70
|
| City |
Oslo |
| ZIP/Postal code |
N-0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL8490 |
| Series (2) |
| GSE39126 |
The human AlkB homologue 4 interacts with proteins associated with transcription (methylation) |
| GSE39135 |
The human AlkB homologue 4 interacts with proteins associated with transcription |
|
