|
| Status |
Public on Dec 31, 2012 |
| Title |
A098-FU-Low dose |
| Sample type |
RNA |
| |
|
| Source name |
FU-Low dose
|
| Organism |
Homo sapiens |
| Characteristics |
disease status: Vietnamese dengue patients
treated with: low-dose (0.5mg/kg) of prednisolone
sample collection time: follow up; 1 month after illness-onset
tissue: blood
patient id: A098
|
| Treatment protocol |
Patients were randomly treated with placebo, low-dose (0.5mg/kg) or high-dose (2mg/kg) of prednisolone. Maximum 3 (pre-treatment (PRE); post-treatment (POST) and follow up (FU) - 1 month since illness onset) and at least 2 samples (pre-treatment and post-treatment) were collected from each patient.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from blood using PAXgene Blood RNA kit (PreAnalytiX, Switzeland) as instructions of manufacturer.
|
| Label |
Biotin
|
| Label protocol |
RNA was amplified and labeled with biotin using Illumina TotalPrep 96 RNA amplification kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 275
|
| Data processing |
The array intensity values were extracted using GenomeStudio software (v.1.6.0, Illumina Inc., USA) and processed with GeneSpring (v.11.5, Agilent Technologies Inc., USA). First measurements having intensity values less than 5.0 were corrected to 5.0, and then all signals were normalized to the 80th percentile by dividing each value by the 80th percentile value; only genes that were confidently detected in at least 60% of samples in one of the three treatment groups were used for the analysis.
|
| |
|
| Submission date |
Aug 16, 2012 |
| Last update date |
Dec 31, 2012 |
| Contact name |
Nguyen Hanh Tien |
| E-mail(s) |
tiennth@oucru.org
|
| Organization name |
Oxford University Clinical Research Unit
|
| Department |
Dengue group
|
| Street address |
764 Vo Van Kiet
|
| City |
Ho Chi Minh |
| ZIP/Postal code |
70000 |
| Country |
Viet Nam |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE40165 |
Immune correlates of early prednisolone therapy in children and adolescents with dengue |
|
