|
| Status |
Public on Sep 10, 2012 |
| Title |
Female 0 estradiol-3 |
| Sample type |
RNA |
| |
|
| Source name |
postnatal day 33-rat mammary gland
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
diet: 0 µg/kg/day E2
gender: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mammary gland pairs were collected and fresh frozen in liquid nitrogen. Total mammary glands RNA was isolated from the rats using TRI reagent and purified using RNeasy mini columns including on-column Dnase digestion.
|
| Label |
biotin
|
| Label protocol |
First- and second- strand cDNA synthesis (from 8 ug total RNA), biotin-labeled aRNA synthesis, fragmentation of cRNA reactions were performed using Affymetrix protocols. aRNA was synthesized from cDNA using a GeneChip IVT labeling kit (Affymetrix) according to the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
Twenty microgram aRNA was then fragmented in 5X fragmentation buffer for 35 min at 94 °C. aRNAs were hybridized to an individual Affymetrix GeneChip Rat arrays (N = 3/treatment) for 16 h at 45ºC in the hybridization oven set at 60 rpm.
|
| Scan protocol |
The probe array was washed and stained using GeneChip fluidics station 450 and scanned using GeneChip Scanner 3000.
|
| Description |
mammary gene expression
|
| Data processing |
Cel files were generated using GCOS and utilized for analysis using GeneSpring GX 11.5.
|
| |
|
| Submission date |
Sep 09, 2012 |
| Last update date |
Sep 10, 2012 |
| Contact name |
Isabelle Racine Miousse |
| E-mail(s) |
iracinemiousse@uams.edu
|
| Organization name |
UAMS
|
| Street address |
15 Children's Way
|
| City |
Little Rock |
| ZIP/Postal code |
72202 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE40713 |
Mammary Gland Morphology and Gene Expression Signature of Prepubertal Male and Female Rats Following Exposure to Exogenous Estradiol |
|
