Adgra2 adhesion G protein-coupled receptor A2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Adgra2provided by MGI
Official Full Name: adhesion G protein-coupled receptor A2provided by MGI
Primary source: MGI:MGI:1925810
See related: Ensembl:ENSMUSG00000031486 AllianceGenome:MGI:1925810
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Tem5; Gpr124; mKIAA1531; 8430414O08Rik; 9530074E10Rik
Summary: Predicted to enable G protein-coupled receptor activity. Involved in several processes, including endothelial cell migration; positive regulation of canonical Wnt signaling pathway; and sprouting angiogenesis. Acts upstream of or within several processes, including negative regulation of vascular endothelial growth factor signaling pathway; positive regulation of endothelial cell migration; and regulation of establishment of blood-brain barrier. Located in cell surface and plasma membrane. Is expressed in several structures, including cardiovascular system; embryo mesenchyme; limb bud; mesonephros; and nose. Orthologous to human ADGRA2 (adhesion G protein-coupled receptor A2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in ovary adult (RPKM 39.8), limb E14.5 (RPKM 26.2) and 22 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 A2; 8 15.91 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (27575546..27613464)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (27085520..27123436)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

GPR124 induces NLRP3 inflammasome-mediated pyroptosis in endothelial cells during ischemic injury.
Title: GPR124 induces NLRP3 inflammasome-mediated pyroptosis in endothelial cells during ischemic injury.
An integrated model for Gpr124 function in Wnt7a/b signaling among vertebrates.
Title: An integrated model for Gpr124 function in Wnt7a/b signaling among vertebrates.
The WNT7A/WNT7B/GPR124/RECK signaling module plays an essential role in mammalian limb development.
Title: The WNT7A/WNT7B/GPR124/RECK signaling module plays an essential role in mammalian limb development.
experiments indicate that Reck and Gpr124 are part of the cell surface protein complex that transduces Wnt7a- and Wnt7b-specific signals in mammalian CNS Epithelial Cells to promote angiogenesis and regulate the BBB.
Title: Reck and Gpr124 Are Essential Receptor Cofactors for Wnt7a/Wnt7b-Specific Signaling in Mammalian CNS Angiogenesis and Blood-Brain Barrier Regulation.
Gpr124 is an endothelial GPCR specifically required for endothelial Wnt signaling and BBB integrity under pathological conditions in adult mice.
Title: Gpr124 is essential for blood-brain barrier integrity in central nervous system disease.
Vital role for GPR124 is potentiation of WNT7-induced canonical beta-catenin signaling with important implications for understanding and manipulating CNS-specific angiogenesis.
Title: GPR124 functions as a WNT7-specific coactivator of canonical β-catenin signaling.
Gpr124 functions as coactivator of Wnt7a- and Wnt7b-stimulated canonical Wnt signaling via Frizzled receptor and Lrp coreceptor and that Gpr124-stimulated signaling functions in concert with Norrin/Frizzled4 signaling to control CNS vascular development
Title: Gpr124 controls CNS angiogenesis and blood-brain barrier integrity by promoting ligand-specific canonical wnt signaling.
GPR124, an orphan G protein-coupled receptor, is required for CNS-specific vascularization and establishment of the blood-brain barrier.
Title: GPR124, an orphan G protein-coupled receptor, is required for CNS-specific vascularization and establishment of the blood-brain barrier.
TGF-beta stimulates Gpr124 expression which is required for angiogenic sprouting into neural tissues.
Title: Angiogenic sprouting into neural tissue requires Gpr124, an orphan G protein-coupled receptor.
study shows that complete null or endothelial-specific GPR124 deletion resulted in embryonic lethality from CNS-specific angiogenesis arrest in forebrain and neural tube; results demonstrate CNS-specific angiogenesis regulation by an endothelial receptor
Title: Essential regulation of CNS angiogenesis by the orphan G protein-coupled receptor GPR124.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Gene trapped (1)
Targeted (8) 1 citation

General gene information

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Markers

NoName (e-PCR)
- UniSTS:235860

NoName (e-PCR)
- UniSTS:235861

Gpr124 (e-PCR), detects polymorphism
- UniSTS:525537

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables G protein-coupled receptor activity	IEA Inferred from Electronic Annotation more info
enables transmembrane signaling receptor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within angiogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in canonical Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in cell surface receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in central nervous system development	IBA Inferred from Biological aspect of Ancestor more info
involved_in central nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endothelial cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of vascular endothelial growth factor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of canonical Wnt signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of canonical Wnt signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of endothelial cell migration	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of angiogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of chemotaxis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of establishment of blood-brain barrier	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of establishment of blood-brain barrier	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within signal transduction	IEA Inferred from Electronic Annotation more info
involved_in sprouting angiogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within sprouting angiogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sprouting angiogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Wnt signalosome	IBA Inferred from Biological aspect of Ancestor more info
part_of Wnt signalosome	ISO Inferred from Sequence Orthology more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info	PubMed

General protein information

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Preferred Names: adhesion G protein-coupled receptor A2

Names: G protein coupled receptor Gpr124; G-protein coupled receptor 124; tumor endothelial marker 5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_054044.2 → NP_473385.2 adhesion G protein-coupled receptor A2 precursor

See identical proteins and their annotated locations for NP_473385.2

Status: VALIDATED

Source sequence(s)

AF378759, CJ243065

Consensus CDS

CCDS22210.2

UniProtKB/Swiss-Prot

B2RRK0, Q91ZV8

UniProtKB/TrEMBL

J3QMG7

Related

ENSMUSP00000033876.8, ENSMUST00000033876.14

Conserved Domains (8) summary

smart00082
Location:192 → 225

LRRCT; Leucine rich repeat C-terminal domain

cd00116
Location:88 → 166

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

sd00031
Location:88 → 109

LRR_1; leucine-rich repeat [structural motif]

pfam01825
Location:709 → 750

GPS; GPCR proteolysis site, GPS, motif

pfam13855
Location:88 → 144

LRR_8; Leucine rich repeat

cl02422
Location:351 → 417

HRM; Hormone receptor domain

cl11960
Location:257 → 343

Ig; Immunoglobulin domain

cl21561
Location:776 → 944

7tm_4; Olfactory receptor

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

27575546..27613464

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006509223.5 → XP_006509286.3 adhesion G protein-coupled receptor A2 isoform X2

UniProtKB/TrEMBL

J3QMG7

Conserved Domains (7) summary

PHA03307
Location:1090 → 1301

PHA03307; transcriptional regulator ICP4; Provisional

COG4886
Location:118 → 349

LRR; Leucine-rich repeat (LRR) protein [Transcription]

sd00031
Location:130 → 151

LRR_1; leucine-rich repeat [structural motif]

cd15998
Location:806 → 1090

7tmB2_GPR124; G protein-coupled receptor 124, member of the class B2 family of seven-transmembrane G protein-coupled receptors

pfam13855
Location:130 → 186

LRR_8; Leucine rich repeat

pfam01825
Location:751 → 792

GPS; GPCR proteolysis site, GPS, motif

cl02422
Location:393 → 459

HRM; Hormone receptor domain
XM_006509222.5 → XP_006509285.3 adhesion G protein-coupled receptor A2 isoform X1

UniProtKB/TrEMBL

J3QMG7

Conserved Domains (8) summary

PHA03307
Location:1094 → 1305

PHA03307; transcriptional regulator ICP4; Provisional

COG4886
Location:118 → 349

LRR; Leucine-rich repeat (LRR) protein [Transcription]

sd00031
Location:130 → 151

LRR_1; leucine-rich repeat [structural motif]

pfam13855
Location:130 → 186

LRR_8; Leucine rich repeat

cd14964
Location:808 → 833

7tm_GPCRs; TM helix 1 [structural motif]

pfam01825
Location:751 → 792

GPS; GPCR proteolysis site, GPS, motif

cl02422
Location:393 → 459

HRM; Hormone receptor domain

cl28897
Location:806 → 1094

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
XM_006509224.5 → XP_006509287.3 adhesion G protein-coupled receptor A2 isoform X3

UniProtKB/TrEMBL

A0A172Q382

Conserved Domains (7) summary

COG4886
Location:118 → 349

LRR; Leucine-rich repeat (LRR) protein [Transcription]

sd00031
Location:130 → 151

LRR_1; leucine-rich repeat [structural motif]

pfam13855
Location:130 → 186

LRR_8; Leucine rich repeat

cd14964
Location:808 → 833

7tm_GPCRs; TM helix 1 [structural motif]

pfam01825
Location:751 → 792

GPS; GPCR proteolysis site, GPS, motif

cl02422
Location:393 → 459

HRM; Hormone receptor domain

cl28897
Location:806 → 877

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily