Tgm4 transglutaminase 4 (prostate) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tgm4provided by MGI
Official Full Name: transglutaminase 4 (prostate)provided by MGI
Primary source: MGI:MGI:3027002
See related: Ensembl:ENSMUSG00000025787 AllianceGenome:MGI:3027002
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: TGP; Eapa1; 9530008N10Rik
Summary: Predicted to enable protein-glutamine gamma-glutamyltransferase activity. Acts upstream of or within mating plug formation. Predicted to be located in Golgi apparatus. Predicted to be active in collagen-containing extracellular matrix. Is expressed in anterior prostate gland and prostate gland dorsolateral lobe. Orthologous to human TGM4 (transglutaminase 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in lung adult (RPKM 1.8), bladder adult (RPKM 1.8) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 F4; 9 73.56 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (122863806..122896623)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (123034741..123067558)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TGM4 is a prostatic secretory molecule with critical role in male reproduction.
Title: Transglutaminase 4 as a prostate autoantigen in male subfertility.
male mice knockouts for transglutaminase IV (Tgm4) fail to form a copulatory plug, demonstrating that this gene is necessary for plug formation and lending a powerful new genetic tool to begin characterizing plug function
Title: Genetic disruption of the copulatory plug in mice leads to severely reduced fertility.
These studies demonstrate that tranglutaminase 2 independent activity exists in the vasculature and that Tgm1 and tgm4 are expressed in vascular tissues
Title: Vena cava and aortic smooth muscle cells express transglutaminases 1 and 4 in addition to transglutaminase 2.
The androgen-dependent SVS I is exclusively expressed in the luminal epithelium of seminal vesicle; Gln232 and Gln254 are the major TG4-reactive residues on SVS I and and TG4 is much stronger than TG2 to cross-link the glutamine residues in SVS I.
Title: Identification of the major TG4 cross-linking sites in the androgen-dependent SVS I exclusively expressed in mouse seminal vesicle.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1)

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein-glutamine gamma-glutamyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within mating plug formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptide cross-linking	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
is_active_in collagen-containing extracellular matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in collagen-containing extracellular matrix	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein-glutamine gamma-glutamyltransferase 4

Names: TGase-4; experimental autoimmune prostatitis antigen 1; transglutaminase-4

NP_808579.2

EC 2.3.2.13

XP_017168931.1

EC 2.3.2.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_177911.4 → NP_808579.2 protein-glutamine gamma-glutamyltransferase 4

See identical proteins and their annotated locations for NP_808579.2

Status: VALIDATED

Source sequence(s)

AF486627, AK035279

Consensus CDS

CCDS23653.1

UniProtKB/Swiss-Prot

B7ZP44, Q8BZH1, Q8K460

UniProtKB/TrEMBL

A0A0R4J071

Related

ENSMUSP00000026893.5, ENSMUST00000026893.6

Conserved Domains (4) summary

smart00460
Location:248 → 340

TGc; Transglutaminase/protease-like homologues

COG1305
Location:251 → 348

YebA; Transglutaminase-like enzyme, putative cysteine protease [Posttranslational modification, protein turnover, chaperones]

pfam00868
Location:9 → 116

Transglut_N; Transglutaminase family

pfam00927
Location:574 → 670

Transglut_C; Transglutaminase family, C-terminal ig like domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

122863806..122896623

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017313442.2 → XP_017168931.1 protein-glutamine gamma-glutamyltransferase 4 isoform X1

UniProtKB/Swiss-Prot

B7ZP44, Q8BZH1, Q8K460

Conserved Domains (3) summary

smart00460
Location:327 → 419

TGc; Transglutaminase/protease-like homologues

pfam00868
Location:86 → 195

Transglut_N; Transglutaminase family

pfam00927
Location:653 → 749

Transglut_C; Transglutaminase family, C-terminal ig like domain