Nos1 nitric oxide synthase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Nos1provided by RGD
Official Full Name: nitric oxide synthase 1provided by RGD
Primary source: RGD:3184
See related: Ensembl:ENSRNOG00000001130 AllianceGenome:RGD:3184
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: bNOS
Summary: Enables several functions, including ATPase binding activity; nucleotide binding activity; and transition metal ion binding activity. Involved in several processes, including blood vessel diameter maintenance; regulation of cation transmembrane transport; and regulation of neuron death. Located in several cellular components, including azurophil granule; nuclear membrane; and sarcolemma. Part of protein-containing complex. Is active in glutamatergic synapse and postsynaptic density, intracellular component. Used to study several diseases, including acute necrotizing pancreatitis; brain ischemia (multiple); end stage renal disease; impotence; and portal hypertension. Biomarker of several diseases, including brain disease (multiple); cardiomyopathy (multiple); hypertension (multiple); kidney failure (multiple); and retinal disease (multiple). Human ortholog(s) of this gene implicated in Alzheimer's disease; Parkinson's disease; asthma; cystic fibrosis; and hypertrophic pyloric stenosis. Orthologous to human NOS1 (nitric oxide synthase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Adrenal (RPKM 54.3), Muscle (RPKM 48.6) and 4 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 12q16

Exon count:: 32

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	12	NC_086030.1 (44276011..44456371, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	12	NC_051347.1 (38615111..38795492, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	12	NC_005111.4 (44214949..44405530, complement)

Chromosome 12 - NC_086030.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Differential superoxide production in phosphorylated neuronal nitric oxide synthase mu and alpha variants.
Title: Differential superoxide production in phosphorylated neuronal nitric oxide synthase mu and alpha variants.
Downregulation of Preso protects against ischemic/reperfusion-mediated neuronal injury through regulating PSD95-nNOS/YAP pathways.
Title: Downregulation of Preso protects against ischemic/reperfusion-mediated neuronal injury through regulating PSD95-nNOS/YAP pathways.
Inhibition of miR-4640-5p alleviates pulmonary hypertension in chronic obstructive pulmonary disease patients by regulating nitric oxide synthase 1.
Title: Inhibition of miR-4640-5p alleviates pulmonary hypertension in chronic obstructive pulmonary disease patients by regulating nitric oxide synthase 1.
Diabetes Mellitus Alters the Immuno-Expression of Neuronal Nitric Oxide Synthase in the Rat Pancreas.
Title: Diabetes Mellitus Alters the Immuno-Expression of Neuronal Nitric Oxide Synthase in the Rat Pancreas.
Inhibition of nNOS in the paraventricular nucleus of hypothalamus decreases exercise-induced hyperthermia.
Title: Inhibition of nNOS in the paraventricular nucleus of hypothalamus decreases exercise-induced hyperthermia.
Reactions of Recombinant Neuronal Nitric Oxide Synthase with Redox Cycling Xenobiotics: A Mechanistic Study.
Title: Reactions of Recombinant Neuronal Nitric Oxide Synthase with Redox Cycling Xenobiotics: A Mechanistic Study.
Disrupting nNOS-PSD95 Interaction Improves Neurological and Cognitive Recoveries after Traumatic Brain Injury.
Title: Disrupting nNOS-PSD95 Interaction Improves Neurological and Cognitive Recoveries after Traumatic Brain Injury.
Long-Term Suppression of c-Jun and nNOS Preserves Ultrastructural Features of Lower Motor Neurons and Forelimb Function after Brachial Plexus Roots Avulsion.
Title: Long-Term Suppression of c-Jun and nNOS Preserves Ultrastructural Features of Lower Motor Neurons and Forelimb Function after Brachial Plexus Roots Avulsion.
Heat shock protein 90alpha increases superoxide generation from neuronal nitric oxide synthases.
Title: Heat shock protein 90α increases superoxide generation from neuronal nitric oxide synthases.
Heat shock protein 90 enhances the electron transfer between the FMN and heme cofactors in neuronal nitric oxide synthase.
Title: Heat shock protein 90 enhances the electron transfer between the FMN and heme cofactors in neuronal nitric oxide synthase.

Submit: New GeneRIF Correction See all GeneRIFs (351)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH142208 (e-PCR)
- UniSTS:231458

RH135131 (e-PCR)
- UniSTS:218408

GDB:437697 (e-PCR)
- UniSTS:157252

RH94638 (e-PCR)
- UniSTS:119885

AU048529 (e-PCR)
- UniSTS:144979

RH128260 (e-PCR)
- UniSTS:211568

D12Rat112 (e-PCR)
- UniSTS:226453

D12Mco2 (e-PCR)
- UniSTS:239527

BE111456 (e-PCR)
- UniSTS:245680

UniSTS:238074 (e-PCR)
- UniSTS:238074

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATPase binding	IPI Inferred from Physical Interaction more info	PubMed
enables FMN binding	IBA Inferred from Biological aspect of Ancestor more info
enables FMN binding	IDA Inferred from Direct Assay more info	PubMed
enables FMN binding	IEA Inferred from Electronic Annotation more info
enables NADP binding	IDA Inferred from Direct Assay more info	PubMed
enables NADP binding	IEA Inferred from Electronic Annotation more info
enables NADPH binding	IDA Inferred from Direct Assay more info	PubMed
enables cadmium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables calcium-dependent protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables calmodulin binding	IDA Inferred from Direct Assay more info	PubMed
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables flavin adenine dinucleotide binding	IBA Inferred from Biological aspect of Ancestor more info
enables flavin adenine dinucleotide binding	IDA Inferred from Direct Assay more info	PubMed
enables flavin adenine dinucleotide binding	IEA Inferred from Electronic Annotation more info
enables heme binding	IDA Inferred from Direct Assay more info	PubMed
enables heme binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nitric-oxide synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables nitric-oxide synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables nitric-oxide synthase activity	IEA Inferred from Electronic Annotation more info
enables nitric-oxide synthase activity	ISO Inferred from Sequence Orthology more info
enables nitric-oxide synthase activity	TAS Traceable Author Statement more info	PubMed
enables phosphoprotein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables scaffold protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sodium channel regulator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables transmembrane transporter binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in arginine catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in arginine catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in behavioral response to cocaine	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of calcium ion transport	ISO Inferred from Sequence Orthology more info
involved_in cellular response to epinephrine stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to growth factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to mechanical stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of establishment of localization in cell	ISO Inferred from Sequence Orthology more info
involved_in establishment of protein localization	IDA Inferred from Direct Assay more info	PubMed
involved_in female pregnancy	IEP Inferred from Expression Pattern more info	PubMed
involved_in multicellular organismal response to stress	IEA Inferred from Electronic Annotation more info
involved_in multicellular organismal response to stress	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of blood pressure	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of blood pressure	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of calcium ion transport	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cytosolic calcium ion concentration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of hepatic stellate cell contraction	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of insulin secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of iron ion transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of neuron apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of potassium ion transport	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of serotonin uptake	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of vasoconstriction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nitric oxide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in nitric oxide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in nitric oxide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in nitric oxide mediated signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in nitric oxide metabolic process	IEP Inferred from Expression Pattern more info	PubMed
involved_in nitric oxide-cGMP-mediated signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of positive regulation of adenylate cyclase-activating adrenergic receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of guanylate cyclase activity	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of long-term synaptic potentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of peptidyl-serine phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of sodium ion transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of the force of heart contraction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of potassium ion transport	ISO Inferred from Sequence Orthology more info
involved_in regulation of neurogenesis	ISO Inferred from Sequence Orthology more info
involved_in regulation of postsynaptic membrane potential	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of sodium ion transport	ISO Inferred from Sequence Orthology more info
involved_in response to activity	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to estrogen	IDA Inferred from Direct Assay more info	PubMed
involved_in response to ethanol	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to heat	IEA Inferred from Electronic Annotation more info
involved_in response to heat	ISO Inferred from Sequence Orthology more info
involved_in response to hormone	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to hypoxia	IEA Inferred from Electronic Annotation more info
involved_in response to hypoxia	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to hypoxia	ISO Inferred from Sequence Orthology more info
involved_in response to lead ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to lipopolysaccharide	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to lipopolysaccharide	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to nicotine	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to nitric oxide	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to nutrient levels	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to organic cyclic compound	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to peptide hormone	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to vitamin E	IEP Inferred from Expression Pattern more info	PubMed
involved_in retrograde trans-synaptic signaling by nitric oxide	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within striated muscle contraction	ISO Inferred from Sequence Orthology more info
involved_in synaptic signaling by nitric oxide	ISO Inferred from Sequence Orthology more info
involved_in vasodilation	IEA Inferred from Electronic Annotation more info
involved_in vasodilation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vasodilation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within xenobiotic catabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in T-tubule	ISO Inferred from Sequence Orthology more info
located_in Z disc	ISO Inferred from Sequence Orthology more info
located_in azurophil granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in calyx of Held	ISO Inferred from Sequence Orthology more info
located_in caveola	ISO Inferred from Sequence Orthology more info
located_in cell periphery	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in membrane raft	ISO Inferred from Sequence Orthology more info
located_in mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in neuron projection terminus	ISO Inferred from Sequence Orthology more info
located_in nuclear membrane	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perikaryon	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in photoreceptor inner segment	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic density	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic density, intracellular component	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic specialization, intracellular component	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
located_in sarcolemma	IDA Inferred from Direct Assay more info	PubMed
located_in sarcolemma	IEA Inferred from Electronic Annotation more info
located_in sarcolemma	ISO Inferred from Sequence Orthology more info
located_in sarcoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in sarcoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in sarcoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
located_in secretory granule	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: nitric oxide synthase 1

Names: N-NOS; NC-NOS; NOS type I; constitutive NOS; nNOS; neuronal NOS; nitric oxidase synthase; nitric oxide synthase 1, neuronal; nitric oxide synthase, brain; peptidyl-cysteine S-nitrosylase NOS1

NP_434686.2

EC 1.14.13.39

XP_038944987.1

EC 1.14.13.39

XP_038944988.1

EC 1.14.13.39

XP_063127111.1

EC 1.14.13.39

XP_063127112.1

EC 1.14.13.39

XP_063127113.1

EC 1.14.13.39

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_052799.2 → NP_434686.2 nitric oxide synthase 1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000012

UniProtKB/TrEMBL

A0A8I5YCJ4, D3ZEW7

Related

ENSRNOP00000062735.2, ENSRNOT00000066810.4

Conserved Domains (4) summary

cd06202
Location:1030 → 1433

Nitric_oxide_synthase; The ferredoxin-reductase (FNR) like C-terminal domain of the nitric oxide synthase (NOS) fuses with a heme-containing N-terminal oxidase domain. The reductase portion is similar in structure to NADPH dependent cytochrome-450 reductase (CYPOR), having an ...

cd00795
Location:300 → 711

NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...

cd00992
Location:15 → 98

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam00258
Location:757 → 964

Flavodoxin_1

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086030.1 Reference GRCr8

Range

44276011..44456371 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039089060.2 → XP_038944988.1 nitric oxide synthase 1 isoform X1

UniProtKB/TrEMBL

A0A8I5YCJ4

Conserved Domains (4) summary

cd06202
Location:1030 → 1433

Nitric_oxide_synthase; The ferredoxin-reductase (FNR) like C-terminal domain of the nitric oxide synthase (NOS) fuses with a heme-containing N-terminal oxidase domain. The reductase portion is similar in structure to NADPH dependent cytochrome-450 reductase (CYPOR), having an ...

cd00795
Location:300 → 711

NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...

cd00992
Location:15 → 98

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam00258
Location:757 → 964

Flavodoxin_1
XM_039089059.2 → XP_038944987.1 nitric oxide synthase 1 isoform X1

UniProtKB/TrEMBL

A0A8I5YCJ4

Conserved Domains (4) summary

cd06202
Location:1030 → 1433

Nitric_oxide_synthase; The ferredoxin-reductase (FNR) like C-terminal domain of the nitric oxide synthase (NOS) fuses with a heme-containing N-terminal oxidase domain. The reductase portion is similar in structure to NADPH dependent cytochrome-450 reductase (CYPOR), having an ...

cd00795
Location:300 → 711

NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...

cd00992
Location:15 → 98

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam00258
Location:757 → 964

Flavodoxin_1
XM_063271041.1 → XP_063127111.1 nitric oxide synthase 1 isoform X2

UniProtKB/Swiss-Prot

P29476, P70594
XM_063271042.1 → XP_063127112.1 nitric oxide synthase 1 isoform X3

UniProtKB/TrEMBL

A0A8I5YCJ4, D3ZEW7
XM_063271043.1 → XP_063127113.1 nitric oxide synthase 1 isoform X4

UniProtKB/Swiss-Prot

P29476, P70594

UniProtKB/TrEMBL

F1LQL1