Masp2 MBL associated serine protease 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Masp2provided by RGD
Official Full Name: MBL associated serine protease 2provided by RGD
Primary source: RGD:620214
See related: Ensembl:ENSRNOG00000011258 AllianceGenome:RGD:620214
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: MAp19; MASP-2
Summary: Enables serine-type endopeptidase activity. Predicted to be involved in complement activation, lectin pathway. Predicted to be active in extracellular space. Orthologous to human MASP2 (MBL associated serine protease 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Liver (RPKM 632.8), Kidney (RPKM 47.4) and 4 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 5q36

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	5	NC_086023.1 (164319017..164332686)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	5	NC_051340.1 (159035892..159049561)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	5	NC_005104.4 (165415105..165429857)

Chromosome 5 - NC_086023.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Flexibility in MASP-1 and MASP-2 provides insight on lectin pathway activation.
Title: Flexibility in Mannan-Binding Lectin-Associated Serine Proteases-1 and -2 Provides Insight on Lectin Pathway Activation.
MASP-1 probably enhances complement activation triggered by MBP.MASP-2 complexes, but it cannot initiate activation itself
Title: Two mechanisms for mannose-binding protein modulation of the activity of its associated serine proteases.
crystal structure of the MASP-2 CUB1-EGF-CUB2 dimer reveals an elongated structure with a prominent concave surface that is proposed to be the mannose-binding protein-binding site
Title: Crystal structure of the CUB1-EGF-CUB2 region of mannose-binding protein associated serine protease-2.
A lysine residue (Lys56) within the conserved motif is essential for MASP-2 binding and probably mediates the specificity of the ficolin-MASP interaction.
Title: Localization and characterization of the mannose-binding lectin (MBL)-associated-serine protease-2 binding site in rat ficolin-A: equivalent binding sites within the collagenous domains of MBLs and ficolins.
Highly conserved intron sequences. Restricted expression contrasts with the ubiquitous expression of neighboring genes.
Title: Organization of the MASP2 locus and its expression profile in mouse and rat.
Analysis of mutant MBL . MASP-2 complexes assembled from recombinant components shows that reduced complement activation by mutant MBLs is caused by failure to activate MASP-2 efficiently on binding to a mannan-coated surface.
Title: Decoupling of carbohydrate binding and MASP-2 autoactivation in variant mannose-binding lectins associated with immunodeficiency.
MASP-2, C4, and C2 and their activation fragments have roles in complement activation via the lectin pathway
Title: Molecular interactions between MASP-2, C4, and C2 and their activation fragments leading to complement activation via the lectin pathway.

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General gene information

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Markers

Masp2 (e-PCR), detects polymorphism
- UniSTS:471084

Masp2 (e-PCR), detects polymorphism
- UniSTS:164693

RH129791 (e-PCR)
- UniSTS:213083

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calcium-dependent protein binding	IEA Inferred from Electronic Annotation more info
enables calcium-dependent protein binding	ISO Inferred from Sequence Orthology more info
enables complement component C4b binding	IEA Inferred from Electronic Annotation more info
enables complement component C4b binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables peptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables peptidase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables serine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables serine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables serine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info
enables serine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in complement activation	NAS Non-traceable Author Statement more info	PubMed
involved_in complement activation, classical pathway	IEA Inferred from Electronic Annotation more info
involved_in complement activation, lectin pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in complement activation, lectin pathway	IEA Inferred from Electronic Annotation more info
involved_in complement activation, lectin pathway	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in extracellular region	IEA Inferred from Electronic Annotation more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: mannan-binding lectin serine protease 2

Names: mannan-binding lectin serine peptidase 2; mannose binding lectin-associated serine protease-2; mannose-binding protein-associated serine protease 2

NP_742040.1

EC 3.4.21.104

XP_006239462.1

EC 3.4.21.104

XP_006239463.1

EC 3.4.21.104

XP_063144421.1

EC 3.4.21.104

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001429904.1 → NP_001416833.1 mannan-binding lectin serine protease 2 isoform 2 precursor

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005
NM_001429905.1 → NP_001416834.1 mannan-binding lectin serine protease 2 isoform 3 precursor

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/TrEMBL

A0A0G2K392, A6IU76
NM_172043.2 → NP_742040.1 mannan-binding lectin serine protease 2 isoform 1 precursor

See identical proteins and their annotated locations for NP_742040.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/Swiss-Prot

Q9JJP3, Q9JJS8, Q9QX83, Q9QX84, Q9QX85, Q9QX86, Q9QX87, Q9QX88, Q9QX89, Q9QX90, Q9QX91, Q9QXD4, Q9WUZ0

UniProtKB/TrEMBL

A2VCV7, F7FHD3

Related

ENSRNOP00000016317.4, ENSRNOT00000016317.7

Conserved Domains (5) summary

smart00020
Location:443 → 678

Tryp_SPc; Trypsin-like serine protease

cd00041
Location:184 → 295

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cd00190
Location:444 → 681

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

pfam00084
Location:300 → 361

Sushi; Sushi repeat (SCR repeat)

pfam14670
Location:152 → 180

FXa_inhibition; Coagulation Factor Xa inhibitory site

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086023.1 Reference GRCr8

Range

164319017..164332686

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063288351.1 → XP_063144421.1 mannan-binding lectin serine protease 2 isoform X1
XM_006239400.5 → XP_006239462.1 mannan-binding lectin serine protease 2 isoform X2

Conserved Domains (2) summary

cd00041
Location:184 → 295

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

pfam14670
Location:152 → 180

FXa_inhibition; Coagulation Factor Xa inhibitory site
XM_006239401.4 → XP_006239463.1 mannan-binding lectin serine protease 2 isoform X3

Related

ENSRNOP00000072546.2

Conserved Domains (2) summary

cd00041
Location:19 → 136

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

pfam14670
Location:152 → 180

FXa_inhibition; Coagulation Factor Xa inhibitory site