GEO DataSets

(Submitter supplied) The Shumiya cataract rat (SCR) is a model for hereditary cataract. Two-third of these rats develop lens opacity within 10-11-weeks. Onset of cataract is attributed to the synergetic effect of lanosterol synthase (Lss) and farnesyl-diphosphate farnesyltransferase 1 (Fdft1) mutant alleles that lead to cholesterol deficiency in the lenses, which in turn adversely affects lens biology including the growth and differentiation of lens epithelial cells (LECs). more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL17117

2 Samples

Download data: CEL

(Submitter supplied) Prolonged use of glucocorticoids can lead to the formation of a cataract, however the mechanism is not known. We recently reported the presence of the functional glucocorticoid receptor in immortalized cultured mammalian lens epithelial cells (Gupta & Wagner, Invest Ophthalmol Vis Sci 2003), but the short term biological effect of glucocorticoid action in lens epithelial cells is not known. This study seeks to examine glucocorticoid induced changes in global gene expression in LECs. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1353

Platform:

GPL96

12 Samples

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Analysis of cultured immortalized lens epithelial cells (LEC) 4 and 16 hours after treatment with 1 uM dexamethasone, a glucocorticoid steroid hormone. Long-term glucocorticoid use can induce cataract formation. Results identify glucocorticoid targets and short-term effects of glucocorticoid on LECs

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 2 time sets

Platform:

GPL96

Series:

GSE3040

12 Samples

Download data

(Submitter supplied) Mutations of the RNA-granule component TDRD7 (OMIM: 611258) cause pediatric cataract in humans. Here, we applied an integrated approach to elucidate the molecular pathology of cataract in Tdrd7 targeted-knockout (Tdrd7-/-) mice. Tdrd7-/- animals precipitously develop lens fiber cell abnormalities early in life, suggesting a global-level breakdown/mis-regulation of key cellular processes. High-throughput RNA-sequencing followed by iSyTE-integrated bioinformatics-based analysis identified the molecular chaperone and cytoskeletal-modulator, HSPB1 (HSP27), among the high-priority down-regulated candidates in Tdrd7-/- lens. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

4 Samples

Download data: TXT

(Submitter supplied) The purpose of this study was to investigate/characterize age- and sex-dependent changes in transcriptomic profile in a mouse model of cataract surgery that mimics human cataract surgery. This surgery involves the removal of lens fiber cells (LFC) leaving behind the lens capsule and associated lens epithelial cells (LEC). The lens capsule and associated remnant LECs were harvested either following cataract surgery (0 hour) or 24 hours later. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

24 Samples

Download data: TXT, XLSX

(Submitter supplied) Celf1 germline or conditional deletion mouse mutants exhibit fully penetrant lens defects including cataract. To gain insight into gene expression changes underlying these lens defects Differential Gene Expression analysis was performed for lenses obtained from control and Celf1 conditional deletion mutant mice.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: CSV

(Submitter supplied) Celf1 germline or conditional deletion mouse mutants exhibit fully penetrant lens defects including cataract. To gain insight into gene expression changes underlying these lens defects, microarray comparison was performed for lenses obtained from control and Celf1 conditional deletion mutant mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

4 Samples

Download data: CSV

(Submitter supplied) Congenital cataract is one of the leading causes of blindness in children worldwide. About one third of congenital cataracts are caused by genetic defects. Previous studies have identified LSS as a causal gene for congenital cataracts. However, its roles in lens development remains largely unknown. Here, we performed RNA-seq on the lens of WT and Lss mutant (heterozygous and homozygous )at E14.5, identified that cholesterol synthesis signaling pathways were significantly downregulated. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

9 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL17117

19 Samples

Download data: CEL

(Submitter supplied) Analysis of gene expression changes during cortical cataract progression in ICR.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL17117

12 Samples

Download data: CEL

(Submitter supplied) Analysis of gene expression changes during cataract progression.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL17117

7 Samples

Download data: CEL

(Submitter supplied) To understand the global view of dysregulated genes and pathwyas in CRYAAN101D lenses, RNA sequencing of 2 & 4 months old CRYAAWT and CRYAAN101D lenses was carried out.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

4 Samples

Download data: XLSX

(Submitter supplied) Analysis of gene expression changes in galactose-induced cataracts when an Atm inhibitors is added. A total of six samples were analyzed: a sample without induced turbidity (control), three samples in which turbidity was induced by galactose (galactose), and a sample with ATM inhibitor added (KU55933 or AZD0156).

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL17117

6 Samples

Download data: CEL

(Submitter supplied) The addition of Glutamate to the lens induced by galactose eliminated the white clouding formed and showed a therapeutic effect. Genes regulated by Glutamate addition were identified by microarray analysis. A total of seven samples were used in this analysis: two samples without galactose (control), three samples with galactose (galactose) and two samples with Glutamate (Glutamate).

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL17117

7 Samples

Download data: CEL

(Submitter supplied) The addition of 2ME2 (HIF-1 inhibitor) to rat model of galactose-induced cataract decreased opacity and therapeutic effect. Genes regulated by 2ME2 addition were identified by microarray analysis. A total of 7 samples were analyzed: samples with no cataract (Control_Day4, Control_Day6), samples with cataract (Galactose-1, Galactose-2, Galactose-3), samples with decreased lens opacity (2ME2, 2ME2-2)

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL17117

7 Samples

Download data: CEL

(Submitter supplied) We investigated the therapeutic effects of histone acetyltransferase (HAT) inhibitors on cataracts using an ex vivo model of galactose-induced cataracts in the rat lens.Among the tested HAT inhibitors, TH1834 was the only inhibitor that could completely reverse white opacities once formed in the lens. Combination treatment with C646/CPTH2 and CBP30/CPTH2 also had therapeutic effects. To identify the genes regulated by HAT inhibitors, we conducted microarray analysis of treated and untreated cataract samples.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL17117

13 Samples

Download data: CEL

(Submitter supplied) By adding MMS or Bleomycin, a DNA damage inducing agent, to the removed rat lens and culturing it, opacity was formed in the lens. To investigate genes whose expression fluctuates when DNA damage is inflicted and causes lens opacity, microarray analysis was performed.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL17117

5 Samples

Download data: CEL

(Submitter supplied) Identification of genes involved in ocular birth defects remains a challenge. To facilitate the identification of genes associated with cataract, we developed iSyTE (integrated Systems Tool for Eye gene discovery; http://bioinformatics.udel.edu/Research/iSyTE). iSyTE contains microarray gene expression profiles of the mouse embryonic lens as it transitions from the stage of placode invagination to that of vesicle formation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS4452 GDS4453

Platform:

GPL1261

19 Samples

Download data: CEL

Analysis of laser capture microdissected tooth germ tissue from ICR E13.5 embryos and matched whole embryo body (WB, without tooth germ) controls representing stages E11.5, E12.5, and E13.5. Results provide insight into molecular mechanisms underlying tooth development.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 development stage, 2 tissue sets

Platform:

GPL1261

Series:

GSE32334

6 Samples

Download data: CEL

Analysis of lens from ICR embryos at 3 key developmental stages in the transition from E10.5 lens placode invagination to E12.5 lens primary fiber cell differentiation and matched whole embryo body (WB, minus ocular tissue) controls. Results provide insight into molecular basis of lens development.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 development stage, 2 tissue sets

Platform:

GPL1261

Series:

GSE32334

13 Samples

Download data: CEL