GEO DataSets

(Submitter supplied) Bilaterian animals have evolved complex sensory organs comprised of distinct cell types that function coordinately to sense the environment. Each sensory unit has a defined architecture built from component cell types, including sensory cells, non-sensory support cells, and dedicated sensory neurons. Whether this characteristic cellular composition is present in the sensory organs of non-bilaterian animals is unknown. more...

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29855

1 Sample

Download data: H5

(Submitter supplied) PRO-seq experiments in multiple organisms. Also, PRO-seq experiment of Nelfb and Nelfe degron mESC in serum/LIF with or without dTAG-13. 

Organism:

Haloferax mediterranei; Dryas iulia; Nematostella vectensis; Dictyostelium discoideum; Capsaspora owczarzaki; Creolimax fragrantissima; Daphnia pulex; Strongylocentrotus purpuratus; Sphaeroforma arctica; Escherichia coli; Mus musculus; Perkinsus marinus

Type:

Other

12 related Platforms

46 Samples

Download data

(Submitter supplied) Cnidarians are the only non-bilaterian group to evolve ciliated larvae with an apical sensory organ integrated with neurons, possibly homologous to the bilaterians. Within cnidarians, an apical organ with a ciliary tuft is identified only in sea anemones. Whether this apical tuft has evolved independently in anthozoans or alternatively originated in the eumetazoans and was lost independently in specific groups of cnidarians and bilaterians is uncertain.

Organism:

Aurelia aurita; Acropora millepora; Acropora tenuis; Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

4 related Platforms

18 Samples

Download data: FASTA, TXT

(Submitter supplied) We demonstrated that the presence of substrate is effectively required for transverse fission, that animal size does not affect fission rates, and that the position of physal pinching is fixed along the oral-aboral axis of the polyp. Gene expression analyses revealed a role for putative homeobox transcription factors, and components of TGFβ, Notch, and FGF signaling pathways in the transverse fission process.

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23029

6 Samples

Download data: TXT

(Submitter supplied) During early animal evolution, the emergence of axially-polarized segments was central to the diversification of complex bilaterian body plans. Nevertheless, precisely how and when segment polarity pathways arose remains obscure. Here we demonstrate the molecular basis for segment polarization in developing larvae of the pre-bilaterian sea anemone Nematostella vectensis. Utilizing spatial transcriptomics, we first constructed a 3-D gene expression atlas of developing larval segments. more...

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29226 GPL23029

31 Samples

Download data: CSV, H5

(Submitter supplied) Isolated mesenteries from MHC::mCherry Adults were used to generate FACS-sorted RNA sequencing libraries in order to characterize gene use in Cnidarian smooth muscle. Transgenic mCherry expressing cells are compared to the non-expressing cells of the surrounding tissues, and up-regulated genes within the muscle cell population can be identified as important muscle-related gene products.

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23802

4 Samples

Download data: TXT

(Submitter supplied) Transcription factor brachyury plays a key role during mesoderm development in vertebrates. In this study we study study the effect of this transcription factor in diploblastic cnidarian model organism N. vectensis

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL17090 GPL23802

17 Samples

Download data: BED, TXT

(Submitter supplied) Understanding how brains evolved is critical to determine the origin(s) of centralized nervous systems. Brains are patterned along their anteroposterior axis by stripes of gene expression that appear to be conserved, suggesting brains are homologous. However, the striped expression is also part of the deeply conserved anteroposterior axial program. An emerging hypothesis is that similarities in brain patterning are convergent, arising through the repeated co-option of axial programs. more...

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29226

4 Samples

Download data: MTX, RDATA, TSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL23029 GPL23802

8 Samples

Download data: MTX, TSV

(Submitter supplied) A diversity of life cycle stages processed into single-cell RNA-seq libraries in order to characterize cell type diversity and relationships

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29226 GPL23029

16 Samples

Download data: CSV, GTF, MTX, TSV

(Submitter supplied) Sub-adult tissue pieces were processed into single cell RNA sequencing libraries in order to catalog spatial information and cell type distribution

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23029

4 Samples

Download data: MTX, TSV

(Submitter supplied) We wanted to infer the contribution of host vs symbiotic microbiota plasticities on thermal acclimation of the holobiont. We long-term acclimated anymals of the same clonal line to 3 different temperatures (15, 20 and 25°C) and monitored along time the changes in fitness, microbiota composition, and host transcriptome.

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29855

15 Samples

Download data: GFF3, TXT

(Submitter supplied) The spatial transcriptome data has allowed us to explore the differential gene expression among the oral-aboral domains and provided new insights into the apical organ signalling pathways for future mechanistic studies and genes associated with oral/aboral differentiation. This resource identifies numerous candidates for elucidating the functional role of the apical organ and to address the evolution of the metazoan larval nervous system.

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29226

10 Samples

Download data: TXT

(Submitter supplied) In mammals, the cGAS-cGAMP-STING pathway is crucial for sensing viral infection and initiating an anti-viral type I interferon response. cGAS and STING are highly conserved genes that originated in bacteria and are present in most animals. By contrast, interferons only emerged in vertebrates; thus, the function of STING in invertebrates is unclear. Here, we use the STING ligand 2'3'-cGAMP to activate immune responses in a model cnidarian invertebrate, the starlet sea anemone Nematostella vectensis. more...

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29226 GPL29855

33 Samples

Download data: TXT

(Submitter supplied) We performed single-cell transcriptome analysis (using 10x genomics 3' scRNA-seq v3.1 chemistry) in the sea anemone Nematostella vectensis (Cnidaria).

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23802

1 Sample

Download data: RDS

(Submitter supplied) We used bacteria isolated from field samples of Nematostella vectensis to quantify gene expression through comparisons of transcriptomes.

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23802

76 Samples

Download data: TXT

(Submitter supplied) The human transcription factor (TF) CGGBP1 (“CGG Binding Protein”) is conserved only in amniotes, and is believed to derive from the zf-BED and Hermes transposase DNA-binding domains (DBDs) of a hAT DNA transposon. Here, we examine the DNA binding preferences of a wide variety of metazoan CGGBP1-like TFs with this bipartite domain using PBM experiments. The derived motifs are available at ...

Organism:

Aedes aegypti; Culex quinquefasciatus; Latimeria chalumnae; Cyprinus carpio; synthetic construct; Hucho hucho; Amphiprion ocellaris; Labeo rohita; Mola mola; Sphaeramia orbicularis; Anabarilius grahami; Petromyzon marinus; Acipenser ruthenus; Gallus gallus; Erpetoichthys calabaricus; Rhizophagus irregularis; Paramormyrops kingsleyae; Acyrthosiphon pisum; Salmo salar; Fundulus heteroclitus; Sparus aurata; Chrysemys picta; Austrofundulus limnaeus; Anabas testudineus; Amphiprion percula; Salarias fasciatus; Locusta migratoria; Crocodylus porosus; Homo sapiens; Rhodnius prolixus; Rhipicephalus appendiculatus; Kryptolebias marmoratus; Nematostella vectensis; Acanthochromis polyacanthus; Hippocampus comes; Hofstenia miamia

Type:

Other

Platform:

GPL11260

258 Samples

Download data: TXT

(Submitter supplied) Knockdown of NveAGO1 and NveAGO2 in Nematostella results in severe developmental defects. NveAGO1 and NveAGO2 immunoprecipitation reveals subfunctionalization for differential miRNA sorting as well as siRNA specialization.

Organism:

Nematostella vectensis

Type:

Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL23029

30 Samples

Download data: XLSX

(Submitter supplied) Gene expression of Nematostella vectensis from 5 different locations under chronic (30 day) temperature stress

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23802

52 Samples

Download data: TXT

(Submitter supplied) We exposed N. vectensis to three forms of trauma (streptomycin, cytochalasin D, or calcium-free seawater) to determine differences in gene expression during recovery from the trauma. Gene expression was monitored during different time points of recovery for animals exposed to cytochalasin D or calcium-free seawater. Gene expression was monitored following designated times of exposure and after 1 hour of recovery for animals exposed to streptomycin.

Organism:

Nematostella vectensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23802

84 Samples

Download data: TXT