DGKG diacylglycerol kinase gamma [Homo sapiens (human)] - Gene

Summary

Official Symbol: DGKGprovided by HGNC
Official Full Name: diacylglycerol kinase gammaprovided by HGNC
Primary source: HGNC:HGNC:2853
See related: Ensembl:ENSG00000058866 MIM:601854; AllianceGenome:HGNC:2853
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: DAGK3; DGK-GAMMA
Summary: This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Expression: Broad expression in brain (RPKM 8.0), heart (RPKM 3.6) and 15 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 3q27.2-q27.3

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	3	NC_000003.12 (186147201..186362234, complement)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	3	NC_060927.1 (188962845..189177970, complement)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	3	NC_000003.11 (185864990..186080023, complement)

Chromosome 3 - NC_000003.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Endothelial DGKG promotes tumor angiogenesis and immune evasion in hepatocellular carcinoma.
Title: Endothelial DGKG promotes tumor angiogenesis and immune evasion in hepatocellular carcinoma.
Characterization of alpha-synuclein N-terminal domain as a novel cellular phosphatidic acid sensor.
Title: Characterization of α-synuclein N-terminal domain as a novel cellular phosphatidic acid sensor.
Decreased expression of DGKgamma in hepatocellular carcinoma tissues was an independent risk factor for a poor prognosis.
Title: Diacylglycerol kinase γ predicts prognosis and functions as a tumor suppressor by negatively regulating glucose transporter 1 in hepatocellular carcinoma.
Epigenetic silencing of DGKG expression is associated with skin cancer.
Title: Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer.
these results indicate that DGKG is an anionic phospholipid binding protein that preferably interacts with a small highly charged head group that is very close to the glycerol or sphingosine backbone.
Title: Diacylglycerol kinase γ is a novel anionic phospholipid binding protein with a selective binding preference.
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
Title: Implication of genetic variants near NEGR1, SEC16B, TMEM18, ETV5/DGKG, GNPDA2, LIN7C/BDNF, MTCH2, BCDIN3D/FAIM2, SH2B1, FTO, MC4R, and KCTD15 with obesity and type 2 diabetes in 7705 Chinese.
Observational study of gene-disease association. (HuGE Navigator)
Title: Analyses of shared genetic factors between asthma and obesity in children.
the interaction of diacylglycerol kinase gamma with the Src homology 2 and C1 domains of beta2-chimaerin is induced synergistically by Phorbol ester and hydrogen peroxide
Title: Phorbol ester and hydrogen peroxide synergistically induce the interaction of diacylglycerol kinase gamma with the Src homology 2 and C1 domains of beta2-chimaerin.
diacylglycerol kinase gamma regulates macrophage differentiation.
Title: Regulatory role of diacylglycerol kinase gamma in macrophage differentiation of leukemia cells.

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Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association yields new sequence variants at seven loci that associate with measures of obesity. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

WI-7381 (e-PCR)
- UniSTS:36875

NIB1365 (e-PCR)
- UniSTS:43534

G60382 (e-PCR)
- UniSTS:137488

RH120077 (e-PCR)
- UniSTS:133400

G09397 (e-PCR)
- UniSTS:58406

SHGC-77629 (e-PCR)
- UniSTS:39980

D3S1602 (e-PCR), detects polymorphism
- UniSTS:12095

G30123 (e-PCR)
- UniSTS:16969

SHGC-77630 (e-PCR)
- UniSTS:31070

WI-15878 (e-PCR)
- UniSTS:46438

SHGC-3840 (e-PCR)
- UniSTS:90472

SHGC-30409 (e-PCR)
- UniSTS:60778

SHGC-77620 (e-PCR)
- UniSTS:1131

SHGC-77631 (e-PCR)
- UniSTS:39536

D3S1617 (e-PCR), detects polymorphism
- UniSTS:39046

D3S1617 (e-PCR), detects polymorphism
- UniSTS:40851

SHGC-77615 (e-PCR)
- UniSTS:4003

DGKG_587 (e-PCR)
- UniSTS:277161

G10718 (e-PCR)
- UniSTS:58034

SHGC-77607 (e-PCR)
- UniSTS:66771

RH120642 (e-PCR)
- UniSTS:132583

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent diacylglycerol kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent diacylglycerol kinase activity	TAS Traceable Author Statement more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables lipid binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in diacylglycerol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in diacylglycerol metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glycerolipid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of protein kinase C signaling	ISS Inferred from Sequence or Structural Similarity more info
involved_in neuron development	IEA Inferred from Electronic Annotation more info
involved_in phosphatidic acid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidic acid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in platelet activation	TAS Traceable Author Statement more info
involved_in protein kinase C-activating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in regulation of dendrite development	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	TAS Traceable Author Statement more info

General protein information

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Preferred Names: diacylglycerol kinase gamma

Names: DAG kinase gamma; diacylglycerol kinase, gamma 90kDa; diacylglyerol kinase gamma; diglyceride kinase gamma

NP_001074213.1

EC 2.7.1.107

NP_001074214.1

EC 2.7.1.107

NP_001337.2

EC 2.7.1.107

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001080744.2 → NP_001074213.1 diacylglycerol kinase gamma isoform 2

See identical proteins and their annotated locations for NP_001074213.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a protein (isoform 2) that is shorter than isoform 1.

Source sequence(s)

AC112649, AC117414, BC089411, BM669549

Consensus CDS

CCDS43182.1

UniProtKB/TrEMBL

Q2M589

Related

ENSP00000339777.4, ENST00000344484.8

Conserved Domains (7) summary

smart00109
Location:336 → 377

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00045
Location:553 → 727

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:436 → 532

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:272 → 321

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:179 → 248

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam13499
Location:180 → 244

EF-hand_7; EF-hand domain pair

pfam14513
Location:5 → 175

DAG_kinase_N; Diacylglycerol kinase N-terminus
NM_001080745.2 → NP_001074214.1 diacylglycerol kinase gamma isoform 3

Status: REVIEWED

Description

Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1, resulting in a protein (isoform 3) that is shorter than isoform 1.

Source sequence(s)

AC112649, AC117414, BC089411, BC112363, BM669549

Consensus CDS

CCDS43181.1

UniProtKB/TrEMBL

Q2M589

Related

ENSP00000371599.4, ENST00000382164.8

Conserved Domains (6) summary

smart00045
Location:539 → 713

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:397 → 518

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:272 → 321

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:179 → 248

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam13499
Location:180 → 244

EF-hand_7; EF-hand domain pair

pfam14513
Location:5 → 175

DAG_kinase_N; Diacylglycerol kinase N-terminus
NM_001346.3 → NP_001337.2 diacylglycerol kinase gamma isoform 1

See identical proteins and their annotated locations for NP_001337.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AC112649, AC117414, BC089411, BM669549, D26135

Consensus CDS

CCDS3274.1

UniProtKB/Swiss-Prot

B2RAH4, P49619, Q2M1H4, Q5FWG1

UniProtKB/TrEMBL

Q2M589

Related

ENSP00000265022.3, ENST00000265022.8

Conserved Domains (7) summary

smart00109
Location:336 → 377

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00045
Location:578 → 752

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:436 → 557

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:272 → 321

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00051
Location:179 → 248

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam13499
Location:180 → 244

EF-hand_7; EF-hand domain pair

pfam14513
Location:5 → 175

DAG_kinase_N; Diacylglycerol kinase N-terminus