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    ARHGAP25 Rho GTPase activating protein 25 [ Homo sapiens (human) ]

    Gene ID: 9938, updated on 3-Apr-2024

    Summary

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    Official Symbol
    ARHGAP25provided by HGNC
    Official Full Name
    Rho GTPase activating protein 25provided by HGNC
    Primary source
    HGNC:HGNC:28951
    See related
    Ensembl:ENSG00000163219 MIM:610587; AllianceGenome:HGNC:28951
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    KAIA0053; HEL-S-308
    Summary
    ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]
    Expression
    Broad expression in lymph node (RPKM 20.1), spleen (RPKM 18.9) and 23 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    Location:
    2p13.3
    Exon count:
    15
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 2 NC_000002.12 (68710544..68826833)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 2 NC_060926.1 (68722999..68839279)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (68961943..69053965)

    Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene transmembrane and coiled-coil domain family 1 pseudogene Neighboring gene prokineticin receptor 1 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:68906264-68906888 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:68906889-68907513 Neighboring gene Sharpr-MPRA regulatory region 5025 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15958 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15959 Neighboring gene NANOG hESC enhancer GRCh37_chr2:68971645-68972146 Neighboring gene uncharacterized LOC105374790 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15960 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15961 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15962 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15963 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15964 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:69042669-69043169 Neighboring gene CRISPRi-validated cis-regulatory element chr2.2482 Neighboring gene long intergenic non-protein coding RNA 1890 Neighboring gene long intergenic non-protein coding RNA 1888 Neighboring gene ReSE screen-validated silencer GRCh37_chr2:69098345-69098505 Neighboring gene bone morphogenetic protein 10

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. A novel BRET-Based GAP assay reveals phosphorylation-dependent regulation of the RAC-specific GTPase activating protein ARHGAP25. Wisniewski É, et al. FASEB J, 2022 Nov. PMID 36190314
    2. ARHGAP25: A negative regulator of colorectal cancer (CRC) metastasis via the Wnt/β-catenin pathway. Tao L, et al. Eur J Pharmacol, 2019 Sep 5. PMID 31228451
    3. Relationship between the expression of ARHGAP25 and RhoA in non-small cell lung cancer and vasculogenic mimicry. Shi F, et al. BMC Pulm Med, 2022 Oct 7. PMID 36207695, Free PMC Article
    4. ARHGAP25 Inhibits Pancreatic Adenocarcinoma Growth by Suppressing Glycolysis via AKT/mTOR Pathway. Huang WK, et al. Int J Biol Sci, 2021. PMID 33994864, Free PMC Article
    5. Identification and characterization of ARHGAP24 and ARHGAP25 genes in silico. Katoh M, et al. Int J Mol Med, 2004 Aug. PMID 15254788

    See all (17) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. ARHGAP25 suppresses the development of breast cancer by an ARHGAP25/Wnt/ASCL2 feedback loop.
      Title: ARHGAP25 suppresses the development of breast cancer by an ARHGAP25/Wnt/ASCL2 feedback loop.
    2. Relationship between the expression of ARHGAP25 and RhoA in non-small cell lung cancer and vasculogenic mimicry.
      Title: Relationship between the expression of ARHGAP25 and RhoA in non-small cell lung cancer and vasculogenic mimicry.
    3. A novel BRET-Based GAP assay reveals phosphorylation-dependent regulation of the RAC-specific GTPase activating protein ARHGAP25.
      Title: A novel BRET-Based GAP assay reveals phosphorylation-dependent regulation of the RAC-specific GTPase activating protein ARHGAP25.
    4. ARHGAP25 Inhibits Pancreatic Adenocarcinoma Growth by Suppressing Glycolysis via AKT/mTOR Pathway.
      Title: ARHGAP25 Inhibits Pancreatic Adenocarcinoma Growth by Suppressing Glycolysis via AKT/mTOR Pathway.
    5. ARHGAP25 negatively regulates the metastatic potential of CRC cells via the Wnt/beta-catenin pathway
      Title: ARHGAP25: A negative regulator of colorectal cancer (CRC) metastasis via the Wnt/β-catenin pathway.
    6. Observational study of gene-disease association. (HuGE Navigator)
      Title: Mutation of ARHGAP9 in patients with coronary spastic angina.
    Submit: New GeneRIF Correction

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    EBI GWAS Catalog

    Description
    Genome-wide association study on dimethylarginines reveals novel AGXT2 variants associated with heart rate variability but not with overall mortality.
    EBI GWAS Catalog

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables GTPase activator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in actin filament organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in actin filament organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in activation of GTPase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in negative regulation of small GTPase mediated signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in negative regulation of small GTPase mediated signal transduction IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in phagocytosis, engulfment IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in phagocytosis, engulfment IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in signal transduction IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in phagocytic cup IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in phagocytic cup IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    rho GTPase-activating protein 25
    Names
    epididymis secretory protein Li 308
    epididymis secretory sperm binding protein
    rho-type GTPase-activating protein 25

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001007231.3 → NP_001007232.2  rho GTPase-activating protein 25 isoform a

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).
      Source sequence(s)
      AC097495, AK290396, BC032222, CD695161
      Consensus CDS
      CCDS33214.2
      UniProtKB/TrEMBL
      A0A2X0TVS2
      Related
      ENSP00000386911.3, ENST00000409202.8
      Conserved Domains (3) summary
      cd04390
      Location:156 → 354
      RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
      cd13263
      Location:45 → 157
      PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain
      pfam00169
      Location:47 → 149
      PH; PH domain

    2. NM_001166276.2 → NP_001159748.1  rho GTPase-activating protein 25 isoform c

      See identical proteins and their annotated locations for NP_001159748.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (c) is shorter and has a distinct N-terminus, compared to isoform a.
      Source sequence(s)
      BC032222
      Consensus CDS
      CCDS54364.1
      UniProtKB/TrEMBL
      A0A2X0TVS2
      Related
      ENSP00000386241.1, ENST00000409220.5
      Conserved Domains (3) summary
      cd04390
      Location:149 → 347
      RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
      cd13263
      Location:38 → 150
      PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain
      pfam00169
      Location:40 → 142
      PH; PH domain

    3. NM_001166277.2 → NP_001159749.1  rho GTPase-activating protein 25 isoform d

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4) uses an alternate in-frame splice site and lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (d) that is shorter than isoform a.
      Source sequence(s)
      AC097495, AK297056, BC032222, CD695161
      Consensus CDS
      CCDS54363.1
      UniProtKB/TrEMBL
      A0A2X0TVS2
      Related
      ENSP00000420583.1, ENST00000467265.5
      Conserved Domains (3) summary
      cd04390
      Location:117 → 314
      RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
      pfam00169
      Location:47 → 115
      PH; PH domain
      cl17171
      Location:45 → 120
      PH-like; Pleckstrin homology-like domain

    4. NM_001364819.1 → NP_001351748.1  rho GTPase-activating protein 25 isoform e

      Status: VALIDATED

      Source sequence(s)
      AC097495, AK290396, BC032222, CD695161, DA462915
      UniProtKB/Swiss-Prot
      A8K2Y1, B7Z498, E9PFQ7, G5E9G2, P42331, Q8IXQ2
      UniProtKB/TrEMBL
      A0A2X0TVS2, A0A384MTM1
      Conserved Domains (3) summary
      cd04390
      Location:155 → 353
      RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
      cd13263
      Location:45 → 155
      PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain
      cl25732
      Location:544 → 644
      SMC_N; RecF/RecN/SMC N terminal domain

    5. NM_001364820.1 → NP_001351749.1  rho GTPase-activating protein 25 isoform f

      Status: VALIDATED

      Source sequence(s)
      AC097495, AK297056, BC032222, BX340497, CD695161
      UniProtKB/TrEMBL
      A0A2X0TVS2
      Conserved Domains (3) summary
      cd04390
      Location:117 → 315
      RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
      cl17171
      Location:45 → 122
      PH-like; Pleckstrin homology-like domain
      cl25732
      Location:506 → 606
      SMC_N; RecF/RecN/SMC N terminal domain

    6. NM_001364821.1 → NP_001351750.1  rho GTPase-activating protein 25 isoform g

      Status: VALIDATED

      Source sequence(s)
      AC097495, AK297056, BC032222, CR991724, DB243756
      UniProtKB/TrEMBL
      A0A2X0TVS2
      Conserved Domains (3) summary
      cd04390
      Location:130 → 328
      RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
      cd13263
      Location:19 → 131
      PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain
      cl25732
      Location:519 → 619
      SMC_N; RecF/RecN/SMC N terminal domain

    7. NM_014882.3 → NP_055697.1  rho GTPase-activating protein 25 isoform b

      See identical proteins and their annotated locations for NP_055697.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (b) is shorter and has a distinct N-terminus, compared to isoform a.
      Source sequence(s)
      BC032222, D29642
      Consensus CDS
      CCDS46312.1
      UniProtKB/TrEMBL
      A0A2X0TVS2, V9HWC8
      Related
      ENSP00000386863.3, ENST00000409030.7
      Conserved Domains (3) summary
      cd04390
      Location:148 → 346
      RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
      cd13263
      Location:38 → 148
      PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain
      pfam00169
      Location:40 → 142
      PH; PH domain

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000002.12 Reference GRCh38.p14 Primary Assembly

      Range
      68710544..68826833
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011533207.4 → XP_011531509.1  rho GTPase-activating protein 25 isoform X1

      See identical proteins and their annotated locations for XP_011531509.1

      UniProtKB/TrEMBL
      A0A2X0TVS2
      Conserved Domains (3) summary
      cd04390
      Location:130 → 328
      RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
      cd13263
      Location:19 → 131
      PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain
      cl25732
      Location:519 → 619
      SMC_N; RecF/RecN/SMC N terminal domain

    2. XM_011533209.3 → XP_011531511.1  rho GTPase-activating protein 25 isoform X2

      UniProtKB/TrEMBL
      H7C4P1
      Related
      ENSP00000417818.1, ENST00000497259.5
      Conserved Domains (1) summary
      cd04390
      Location:38 → 236
      RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...

    3. XM_017005426.2 → XP_016860915.1  rho GTPase-activating protein 25 isoform X3

      UniProtKB/TrEMBL
      C9JB56
      Conserved Domains (1) summary
      cl02570
      Location:1 → 80
      RhoGAP; RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when ...

    4. XM_011533210.4 → XP_011531512.1  rho GTPase-activating protein 25 isoform X3

      UniProtKB/TrEMBL
      C9JB56
      Related
      ENSP00000417467.1, ENST00000479844.1
      Conserved Domains (1) summary
      cl02570
      Location:1 → 80
      RhoGAP; RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when ...

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060926.1 Alternate T2T-CHM13v2.0

      Range
      68722999..68839279
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054344775.1 → XP_054200750.1  rho GTPase-activating protein 25 isoform X1

    2. XM_054344776.1 → XP_054200751.1  rho GTPase-activating protein 25 isoform X2

    3. XM_054344777.1 → XP_054200752.1  rho GTPase-activating protein 25 isoform X3

    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P42331.2 GenPept UniProtKB/Swiss-Prot:P42331

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