|
| Status |
Public on Oct 09, 2013 |
| Title |
iron deficient control littermates, LPS injected 3 |
| Sample type |
RNA |
| |
|
| Source name |
mouse liver_iron deficient control littermates, LPS injected
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed 129/Ola × C57BL/6
tissue: liver
genotype/variation: wild type
growth protocol: 4 week old mice maintained on iron deficient diet for 3 weeks
stress: LPS
age: 7 weeks old
|
| Treatment protocol |
Mice were injected with LPS (from Escherichia coli O26:B6; 0.1 mg/kg) or saline solution, and sacrificed 6 hrs later.
|
| Growth protocol |
4 weeks old mice were maintained an iron replete (Tmprss6 KO mice) or an iron deficient (control littermates) diet for 3 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total liver RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Total RNA was first reverse transcribed into cDNA and then in vitro trascribed into biotin-UTP labelled cRNA using the Illumina TotalPrep RNA Amplification Kit (Ambion, Austin, TX)
|
| |
|
| Hybridization protocol |
750 ng of cRNA in 11 µl (150 ng/µl) were hybridized to the BeadChip Array at 58°C overnight. The fluorescent signal was developed through a streptavidin-Cy3 staining step.
|
| Scan protocol |
Beadchips were imaged using the Illumina BeadArray Reader; the software Illumina® GenomeStudio was used to take fluorescent hybridization signals.
|
| Description |
ID+LPS-3.AVG_Signal
experiment replicate 3
|
| Data processing |
The data were normalized using the cubic spline algorith implemented in the Illumina® GenomeStudio software
|
| |
|
| Submission date |
Apr 22, 2013 |
| Last update date |
Oct 09, 2013 |
| Contact name |
Michela Riba |
| E-mail(s) |
riba.michela@gmail.com
|
| Organization name |
IRCCS, Ospdale San Raffaele
|
| Department |
COSR, Center for Omics Sciences
|
| Street address |
via Olgettina 60
|
| City |
Milano |
| ZIP/Postal code |
20032 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE46287 |
A strong anti-inflammatory signature revealed by liver transcription profiling of Tmprss6-/- mice |
|
