|
| Status |
Public on Oct 31, 2013 |
| Title |
Primary AML sample - 14 |
| Sample type |
RNA |
| |
|
| Source name |
AML Patient
|
| Organism |
Homo sapiens |
| Characteristics |
phenotype: Leukemia
tissue: Primary AML sample
|
| Treatment protocol |
N/A
|
| Growth protocol |
All the leukemia cell lines were culture in vitro under appropriate cell cutlure conditions according to the supplier recommondataion. Normal mature hematopoietic cells were purifed from normal healthy donor peripheral blood using Ficoll gradiant centrifugation followed by FACS sorting.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell were lysed in Trizol and total RNA was purifed using miRNAeasy kit according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
About 500 ng of total RNA was biotin labeled using Affymetrix propritary FlashTag Biotin HSR RNA labeling Kit that is covalently linked to biotin.
|
| |
|
| Hybridization protocol |
Biotin labled RNA was hybridized for 16 hr at 45C on GeneChip miRNA 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Genechip Scanner 3000 and software AffyMetrix Genechip Command Console .
|
| Description |
miRNA Expression Data from AML Patient
|
| Data processing |
The data were analyzed using the Partek Genomic Suite 6.6 to load the data before using the RMA algorithem to normalize all of the data
|
| |
|
| Submission date |
Oct 30, 2013 |
| Last update date |
Oct 31, 2013 |
| Contact name |
Amol Carl Shetty |
| E-mail(s) |
ashetty@som.umaryland.edu
|
| Organization name |
University of Maryland Baltimore
|
| Department |
Institute for Genome Sciences
|
| Street address |
670 W. Baltimore Street, Floor 3
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21201 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE51908 |
miRNA expression data from normal and Malignant hematopoietic cells |
|
