|
| Status |
Public on Nov 24, 2014 |
| Title |
Naïve MG-SC |
| Sample type |
RNA |
| |
|
| Source name |
Mouse -B6/SJL-miR-155+/-
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Spinal FACS sorted adult FCRLS+ microglia
strain/genotype: B6SJL-Non-Tg/miR-155+/-
gender: female
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using mirVanaTM miRNA isolation kit (Ambion) according to the manufacturer’s protocol
|
| Label |
NA
|
| Label protocol |
nCounter Mouse Inflammation Kit
|
| |
|
| Hybridization protocol |
according to the manufacturer’s protocol
|
| Scan protocol |
according to the manufacturer’s protocol
|
| Data processing |
Data was proccessed using nSolver, normalized to Positive controls, Housekeeping genes and negative controls
|
| |
|
| Submission date |
Nov 22, 2013 |
| Last update date |
Nov 24, 2014 |
| Contact name |
Oleg Butovsky |
| E-mail(s) |
obutovsky@rics.bwh.harvard.edu
|
| Phone |
1-617-525-5313
|
| Organization name |
Brigham and Women's Hospital, Harvard Medical School
|
| Department |
Center of Neurologic Diseases
|
| Lab |
Dr. Oleg Butovsky
|
| Street address |
77 Avenue Louis Pasteur, Office 614
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL17981 |
| Series (2) |
| GSE52673 |
Inflammation gene profile of sc-microglia in SOD mice |
| GSE52947 |
Targeting miR-155 restores abnormal microglia and attenuates disease in SOD1 mice |
|
