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| Status |
Public on Nov 24, 2014 |
| Title |
Inflammation gene profile of sc-microglia in SOD mice |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
Amyotrophic lateral sclerosis (ALS) is a paralytic degenerative disease of the nervous system. In the SOD1 mouse model of ALS we found loss of the molecular and functional microglia signature associated with pronounced expression of miR-155 in SOD1 mice. We also found increased expression of miR-155 in the spinal cord of ALS subjects. Genetic ablation of miR-155 increased survival in SOD1 mice and reversed the abnormal microglial and monocyte molecular signature. In addition, dysregulated proteins in the spinal cord of SOD1 mice that we identified in human ALS spinal cords and CSF were restored in SOD1G93A/miR155-/- mice. Treatment of SOD1 mice with anti-miR-155 SOD1 mice injected systemically or into the cerebrospinal fluid prolonged survival and restored the microglial unique genetic and microRNA profiles. Our findings provide a new avenue for immune based therapy of ALS by targeting miR-155.
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| Overall design |
Total RNA was isolated from FACS sorted adult FCRLS+ microglia from spinal cords and Ly6CHi splenic monocytes from Non-Tg/miR155+/-, SOD1G93A-miR155+/- and SOD1G93A-miR155–/- mice at the age of 120d. Total RNA was extracted using mirVanaTM miRNA isolation kit (Ambion) according to the manufacturer’s protocol. nCounter Mouse inflammation kit was used for gene expression profile.
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| Contributor(s) |
Butovsky O |
| Citation(s) |
25381879 |
| Submission date |
Nov 22, 2013 |
| Last update date |
Nov 26, 2014 |
| Contact name |
Oleg Butovsky |
| E-mail(s) |
obutovsky@rics.bwh.harvard.edu
|
| Phone |
1-617-525-5313
|
| Organization name |
Brigham and Women's Hospital, Harvard Medical School
|
| Department |
Center of Neurologic Diseases
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| Lab |
Dr. Oleg Butovsky
|
| Street address |
77 Avenue Louis Pasteur, Office 614
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL17981 |
Nanostring mouse Inflammation kit |
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| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE52947 |
Targeting miR-155 restores abnormal microglia and attenuates disease in SOD1 mice |
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| Relations |
| BioProject |
PRJNA230489 |
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