|
| Status |
Public on Sep 08, 2014 |
| Title |
Hippocampus_rat Minimal Behavioral Response_rat 2R |
| Sample type |
RNA |
| |
|
| Source name |
Rat Hippocampus
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
age: Adult
tissue: Hippocampus
gender: male
group: Minimal Behavioral Response
rat i.d.: 2R
|
| Treatment protocol |
Hippocampus was dissected on dry ice using established anatomical landmarks. Tissues were immediately submerged in RNA-stabilization solution (RNAlater, Ambion), and stored at −80 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kits (Qiagen) were used to isolate total RNA with the addition of on-column DNase digestion. Quality control was performed with ND-1000 spectrophotometer (NanoDrop Technologies) and 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with Illumina TotalPrep RNA Amplification Kit (Ambion).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
We retained probes for which the majority (>50%) of samples from each group displayed a detection P value lower than <0.05.
|
| |
|
| Submission date |
Aug 11, 2014 |
| Last update date |
Sep 08, 2014 |
| Contact name |
Nikolaos Daskalakis |
| E-mail(s) |
ndaskalakis@mclean.harvard.edu
|
| Phone |
6178552785
|
| Organization name |
McLean Hospital/ Harvard Medical School
|
| Department |
Department of Psychiatry
|
| Lab |
Neurogenomics & Translational Bioinformatics Laboratory
|
| Street address |
Oaks Building 328, Mailstop 212, 115 Mill Street
|
| City |
Belmont |
| State/province |
MA |
| ZIP/Postal code |
02478 |
| Country |
USA |
| |
|
| Platform ID |
GPL6101 |
| Series (2) |
| GSE60303 |
Genome-wide analysis of stress-exposure-associated and exposure-related individual differences associated hippocampus gene expression in males and females. |
| GSE60304 |
PTSD model |
|
