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Status
Public on Dec 11, 2007
Title
Day 1 control 7
Sample type
RNA
Source name
rat lung, day1, ctrl
Organism
Rattus norvegicus Characteristics
Strain : SD
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
Hybridization protocol
Standard Affymetrix protocol
Scan protocol
Standard Affymetrix protocol
Description
rat lung, day1, ctrl
Data processing
The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
Submission date
Feb 20, 2007
Last update date
Dec 11, 2007
Contact name
Christopher S Stevenson
Organization name
Novartis Institutes for BioMedical Research
Department
Respiratory Diseases
Street address
Wimblehurst Road
City
Horsham
ZIP/Postal code
RH12 5AB
Country
United Kingdom
Platform ID
GPL85 Series (1)
GSE7079 Chronic rat exposure to cigarette smoke
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-3_at
187.400000
P
0.000070
AFFX-BioB-3_st
6.800000
A
0.814869
AFFX-BioB-5_at
109.200000
P
0.002556
AFFX-BioB-5_st
33.500000
A
0.078002
AFFX-BioB-M_at
244.800000
P
0.000044
AFFX-BioB-M_st
12.700000
A
0.470241
AFFX-BioC-3_at
315.300000
P
0.000044
AFFX-BioC-3_st
2.600000
A
0.772364
AFFX-BioC-5_at
508.800000
P
0.000081
AFFX-BioC-5_st
2.900000
A
0.927300
AFFX-BioDn-3_at
2046.000000
P
0.000060
AFFX-BioDn-3_st
24.200000
A
0.185131
AFFX-BioDn-5_at
386.400000
P
0.000070
AFFX-BioDn-5_st
36.100000
A
0.156732
AFFX-CreX-3_at
6039.900000
P
0.000044
AFFX-CreX-3_st
3.600000
A
0.500000
AFFX-CreX-5_at
2948.100000
P
0.000044
AFFX-CreX-5_st
10.600000
A
0.216524
AFFX-DapX-3_at
6.500000
A
0.737173
AFFX-DapX-5_at
6.700000
A
0.327079
8799 295 Kbytes .Supplementary file Size Download File type/resource GSM170341.cel.gz
1.9 Mb
(ftp) (http) CEL
Processed data included within Sample table
