|
| Status |
Public on Sep 28, 2016 |
| Title |
JJN3_CPI 0.01uM + LENA 5uM/Dexa 0.05uM 6h |
| Sample type |
RNA |
| |
|
| Source name |
JJN3 multiple myeloma cell line, COMBO
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: JJN3
cell type: multiple myeloma cell line
treatment: CPI203 plus lenalidomide plus dexamethasone
|
| Treatment protocol |
MM cells were treated with 0.01uM CPI203 +/- 5uM LENA and 0.05uM Dexa for 6h.
|
| Growth protocol |
MM cells at exponential phase were set at 5x10^5 cells/ml for the experiment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Trizol reagent.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 150ng total RNA.
|
| |
|
| Hybridization protocol |
RNA samples were processed to Affymetrix GeneChip HT HG-U219 arrays.
|
| Scan protocol |
Scanning was processed in the GeneTitan instrument.
|
| Description |
JJN3_COMBO
Gene expression data in treated JJN3 cells.
|
| Data processing |
Determination of the signal value for each probe set of the array were obtained with GeneChip® Command Console® Software (AGCC) (Affymetrix).
Raw data were normalized using the Robust Multichip Analysis (RMA) algorithm implemented in the Expression Console Software v1.1 (Affymetrix).
|
| |
|
| Submission date |
Sep 27, 2016 |
| Last update date |
Sep 28, 2016 |
| Contact name |
Patricia Pérez-Galán |
| E-mail(s) |
pperez@clinic.cat
|
| Phone |
932275400
|
| Organization name |
IDIBAPS
|
| Department |
Hemato-oncology
|
| Street address |
Centre Esther Koplowitz, Hemato-oncology dept, Rosello 149-153, 2nd floor
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE87403 |
The BET bromodomain inhibitor CPI203 improves lenalidomide activity in in vitro and in vivo models of multiple myeloma by synergistic blockade of Ikaros and c-Myc signaling |
|
