|
| Status |
Public on May 22, 2017 |
| Title |
PHH_Aflatoxin_7d_2 |
| Sample type |
RNA |
| |
|
| Source name |
Primary human hepatocyte 3D spheroid
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Primary human hepatocyte (PHH)
treatment time: 7d
treatment: Aflatoxin B1
|
| Treatment protocol |
Medium supplemented with 0.4% DMSO was changed every 2-3 days. For PHH spheroids cells were also treated with either aflatoxin B1 (0.002uM), amiodarone (4uM) or chlorpromazine (6uM).
|
| Growth protocol |
Primary human hepatocytes were seeded in ultra-low attachment plates at 1500 cells/well in 100ul Williams E basal medium supplemented with 10% FBS and centrifuged at 100g for 2 minutes. From day 4 onwards daily medium changes with FBS-free medium were performed. Treatments were initiated on day 7.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the AllPrep DNA/RNA Mini Kit (Qiagen) according to the manufacturer’s instructions. RNA concentration and quality was assessed using the Agilent Bioanalyzer
|
| Label |
Cy3
|
| Label protocol |
Total RNA samples (45ng) were labelled with Cy3 using the Low Input Quick Amp Labeling Kit–One Colour (Agilent# 5190-2305)
|
| |
|
| Hybridization protocol |
Agilent 8x60K Whole Human Genome Oligo Microarray slides (G4851B) were hybridised, washed and then scanned on an Agilent Microarray Scanner
|
| Scan protocol |
Agilent 8x60K Whole Human Genome Oligo Microarray slides (G4851B) were hybridised, washed and then scanned on an Agilent Microarray Scanner
|
| Description |
Gene expression after 7 day treatment (3 re-dosings) with 0.002uM aflatoxin B1 (0.4% DMSO), PHH 3D spheroid
|
| Data processing |
Images from the Agilent scanner were processed using Agilent Feature Extraction Software v10.7.3.1.
|
| |
|
| Submission date |
Jan 19, 2017 |
| Last update date |
May 22, 2017 |
| Contact name |
Magnus Ingelman-Sundberg |
| E-mail(s) |
Magnus.Ingelman-Sundberg@ki.se
|
| Organization name |
Karolinska Institutet
|
| Street address |
Nanna Svartz väg 2
|
| City |
Stockholm |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE93840 |
Transcriptional comparisons of stem cell-derived hepatocytes (hiPS-Hep), HepaRG cells and 3D human hepatocyte spheroids as predictive in vitro systems for drug-induced liver injury |
|
