|
| Status |
Public on Apr 01, 2008 |
| Title |
UKA Rat 4x44K No. 2 Processed |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Hepatic stellate cells, one animal, transfected with siRNA targeting endoglin
|
| Organism |
Rattus norvegicus |
| Characteristics |
RNA was taken from hepatic stellate cells that were transfected with a siRNA targeting endoglin
|
| Biomaterial provider |
UKA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy
|
| Label |
Cy5
|
| Label protocol |
RNA was labeled using the Agilent Low RNA Input Linear Amp Kit
|
| |
|
| Channel 2 |
| Source name |
Hepatic stellate cells, one animal, transfected with siRNA control
|
| Organism |
Rattus norvegicus |
| Characteristics |
RNA was taken from hepatic stellate cells that were transfected with a control siRNA
|
| Biomaterial provider |
UKA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy
|
| Label |
Cy3
|
| Label protocol |
RNA was labeled using the Agilent Low RNA Input Linear Amp Kit
|
| |
|
| |
| Hybridization protocol |
Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit (Agilent)
|
| Scan protocol |
Agilent's DNA microarray scanner
|
| Description |
Agilent Feature Extraction Software (FES) and further analyzed using the Rosetta Resolver gene expression data analysis system
|
| Data processing |
Agilent Feature Extraction Software (FES)
|
| |
|
| Submission date |
Jan 11, 2008 |
| Last update date |
Jul 17, 2017 |
| Contact name |
Ralf Weiskirchen |
| E-mail(s) |
rweiskirchen@ukaachen.de
|
| Phone |
+49-241-8088683
|
| Fax |
+49-241-8082512
|
| URL |
http://www.ukaachen.de/content/folder/5095184
|
| Organization name |
RWTH University Hospital Aachen
|
| Department |
Institute of Clinical Chemistry and Pathobiochemistry
|
| Street address |
Pauwelsstr. 30
|
| City |
Aachen |
| State/province |
NRW |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4135 |
| Series (1) |
| GSE10148 |
Analysis of Endoglin function in rat hepatic stellate cells (HSC) |
|
