|
| Status |
Public on Apr 11, 2017 |
| Title |
HIV+ NOT IMPAIRED 7200848468 |
| Sample type |
RNA |
| |
|
| Source name |
post mortem human neocortex
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: female
age: 44
|
| Treatment protocol |
N/A
|
| Growth protocol |
Archived post mortem human neocortex
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen miRNeasy serum/plasma kit extraction as per manufacturer's instructions
|
| Label |
Biotin
|
| Label protocol |
8 µg total RNA labelled with Affymetrix FlashTag Biotin HSR kit as per manufacturer's instructions
|
| |
|
| Hybridization protocol |
labeled samples were hybridized to Affymetrix miRNA 3.0 GeneChip array at 48 °C for 16 h
|
| Scan protocol |
Arrays were stained and washed on Affymetrix GeneChip Fluidics 450 following manufacturer's protocol and scanned with Affymetrix GeneChip Scanner 3000 7G System
|
| Description |
miRNA expression data from post mortem human neocortex
|
| Data processing |
GeneSpring (version 12.6, Agilent Technologies) were used to normalize the data based on the Robust Multiarray Average algorithm, in which data are background-corrected, log 2 transformed and quartile normalized
|
| |
|
| Submission date |
Apr 10, 2017 |
| Last update date |
Apr 11, 2017 |
| Contact name |
William George Branton |
| E-mail(s) |
wbranton@ualberta.ca
|
| Phone |
780-492-2148
|
| Organization name |
University of Alberta
|
| Department |
Medicine
|
| Lab |
Dr. C. Power
|
| Street address |
611 HMRC University of Alberta
|
| City |
Edmonton |
| State/province |
Alberta |
| ZIP/Postal code |
T6G2S2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE97611 |
Disrupted miRNA expression in HAND |
|
