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Status |
Public on Sep 14, 2018 |
Title |
Kras spleen NK, replicate 1 |
Sample type |
RNA |
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Source name |
Kras spleen NK
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Organism |
Mus musculus |
Characteristics |
strain background: C57BL/6 genotype/variation: Kras age: 6-8 weeks infected with: Lenti-Cre for induction of lung cancer time point: Stage 3 (16 weeks after Lenti-Cre delivery) tissue: spleen cell type: Natural killer (NK)
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Treatment protocol |
NK cells were isolated from spleens and lungs of WT and Stage 3 Kras mice, and we lysed the cells in TRIzol (Invitrogen) and freezed them in refrigerator (-80°C).
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted and purified using miRNeasy Mini Kit and RNase-Free Dnase Set.Total RNA quality was assessed with a Pico Chip on an Agilent 2100 Bioanalyzer (Agilent Technologies).
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Label |
indocarbocyanine
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Label protocol |
RNA was amplified and abeling of CTP with indocarbocyanine was done with Agilent one-color labeling kits (Agilent Technologies).
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Hybridization protocol |
Indocarbocyanine-labeled cDNA was assessed with the NanoDrop ND-2000 (Thermo Scientific), and equal amounts of indocarbocyanine-labeled target were hybridized to Agilent SurePrint G3 Mouse Gene Expression (8x60K) microarrays(Design ID:74809).
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Scan protocol |
Arrays were scanned with the Agilent Scanner G2505C(Agilent Technologies).
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Description |
SAMPLE 5
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Data processing |
Raw signal intensities were extracted and quantile normalized with Feature Extraction v10.7.1.1 software(Agilent Technologies).
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Submission date |
Sep 14, 2017 |
Last update date |
Sep 14, 2018 |
Contact name |
Shu Zhu |
E-mail(s) |
zhushu@ustc.edu.cn
|
Organization name |
University of Science and Technology of China
|
Street address |
443 Huangshan Road, Hefei, Anhui, China
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City |
Hefei |
ZIP/Postal code |
230027 |
Country |
China |
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Platform ID |
GPL21163 |
Series (1) |
GSE103856 |
Expression data from lung and spleen natural killer cells of WT and Kras mice |
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