|
| Status |
Public on Oct 14, 2017 |
| Title |
C14_12h_con_repC |
| Sample type |
RNA |
| |
|
| Source name |
CEM C7-14_12h_ethanol
|
| Organism |
Homo sapiens |
| Characteristics |
cem clone id: CEM C7-14
cell type: human leukemic cells
time: 12h
treatment: ethanol
|
| Treatment protocol |
Dex in ethanol was added to 10E-6 M Dex and <1% ethanol final. Control cultures received ethanol only.
|
| Growth protocol |
CEM cells were maintained in log growth till the initiation of treatment. Triplicate independent cultures were then placed in tissue culture wells at 2x10E5 cells per ml. growth medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from concentrated, washed cells by use of Qiagen RNA isolaztion kit.
|
| Label |
biotin
|
| Label protocol |
RNA was biotin labeled by use of ENZO Biochem. Trascriptional labeling kit.
|
| |
|
| Hybridization protocol |
Post fragmentation, hybridization was carried out as described (RD Medh, et al. Genomics 2003. 81(6):543-55
|
| Scan protocol |
Gene Array Scanner (Hewlet Packard)
|
| Description |
12hGCC-14con
|
| Data processing |
By Affymetrix Gene Chip Suites 4.0 and 5.0 (the latter in Gene Spring software version 4.2.1)
|
| |
|
| Submission date |
Oct 13, 2017 |
| Last update date |
Oct 14, 2017 |
| Contact name |
Scott Andrew Ochsner |
| E-mail(s) |
sochsner@bcm.edu
|
| Phone |
713-798-6227
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Cellular Biology
|
| Lab |
SPP: Signaling Pathways Project
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL8300 |
| Series (2) |
| GSE104930 |
Sequential gene regulatory events leading to glucocorticoid-evoked apoptosis of CEM human leukemic cells [HG_U95Av2] |
| GSE104932 |
Sequential gene regulatory events leading to glucocorticoid-evoked apoptosis of CEM human leukemic cells |
|
