|
Status |
Public on Dec 09, 2020 |
Title |
201525200072_R08C01 |
Sample type |
genomic |
|
|
Source name |
SJ-DIPGX53 PDX
|
Organism |
Homo sapiens |
Characteristics |
line id: SJ-DIPGX53 methylation classification: DMG_K27
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA was isolated from frozen tumor tissue by SDS/Proteinase K digestion and phenol/chloroform extraction or from FFPE using a QIAamp DNA FFPE Tissue Kit (Qiagen) according to manufacturer's instructions.
|
Label |
Cy3, Cy5
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium MethylationEPIC or HumanMethylation450 BeadChip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Data processing |
BeadStudio software v3.2 CSV files: Average Beta & Unmethylated and methylated signal intensities
|
|
|
Submission date |
Jun 08, 2020 |
Last update date |
Dec 10, 2020 |
Contact name |
Suzanne J. Baker |
E-mail(s) |
Suzanne.Baker@STJUDE.ORG
|
Organization name |
ST. JUDE CHILDREN'S RESEARCH HOSPITAL
|
Street address |
262 Danny Thomas Place
|
City |
MEMPHIS |
State/province |
TENNESSEE |
ZIP/Postal code |
38105 |
Country |
USA |
|
|
Platform ID |
GPL21145 |
Series (1) |
GSE152035 |
Patient-Derived Orthotopic Xenografts and Cell Lines from Pediatric High-Grade Glioma Recapitulate Heterogeneity of Histopathology, Molecular Signatures and Drug Response |
|