|
| Status |
Public on Nov 01, 2020 |
| Title |
Ovarian Clear Cell Carcinoma cell line KOC-7c |
| Sample type |
genomic |
| |
|
| Source name |
Ovarian Clear Cell Carcinoma cell line KOC-7c
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Ovarian Clear Cell Carcinoma cell line
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Cell lines were cultured in Dulbecco’s modified Eagle medium/Ham’s F12 (Wisent) and supplemented with 10% FBS (Wisent). Cells were maintained in at 37 °C and a CO2 concentration of 5%. Cells were allowed to reach ~90% confluency at which point the media was aspirated and cells were washed with phosphate buffered saline (PBS) and exposed to 2 mL of trypsin/EDTA until cells detached from plate. Cells were resuspended in 10 mL of media and then split onto new plates at a minimum of a 1:3 dilution.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Wizard Genomic DNA Purification Kit (Promega) was used to isolate DNA from cells as per manufacturers protocol.
|
| Label |
Biotin
|
| Label protocol |
Affymetrix CytoScan HD Reagent kit
|
| |
|
| Hybridization protocol |
50°C, 60 rpm for 16 hr-18hr
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 (#3)
|
| Description |
Affymetrix CytoScan HD Reagent kit
|
| Data processing |
ChAS
Genotype Call (SNP call)
|
| |
|
| Submission date |
Oct 31, 2020 |
| Last update date |
Nov 01, 2020 |
| Contact name |
Bart Kolendowski |
| Organization name |
Lawson Research
|
| Street address |
750 Base Line Rd E
|
| City |
London |
| State/province |
ONTARIO |
| ZIP/Postal code |
N6C2R5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16131 |
| Series (2) |
| GSE160569 |
Characterization of Mutational Status, Spheroid Formation, and Drug Response of a New Genomically-Stable Human Ovarian Clear Cell Carcinoma Cell Line, 105C (Affymetrix) |
| GSE160571 |
Characterization of Mutational Status, Spheroid Formation, and Drug Response of a New Genomically-Stable Human Ovarian Clear Cell Carcinoma Cell Line, 105C |
|
