|
| Status |
Public on Aug 22, 2011 |
| Title |
Control-2 |
| Sample type |
RNA |
| |
|
| Source name |
liver of rat fed a high-fat diet and high-fructose water without Eucommia ulmoides leaves
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar/ST
gender: male
tissue: liver
|
| Treatment protocol |
Rats fed a high-fat diet and high-fructose water were orally administrated Eucommia ulmoides leaf extract as drinking water. After 5 weeks, rats were anesthetized and killed by exsanguination. Parts of liver of each rat was taken and immediately immersed in RNAlater (Applied Biosystems).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAiso extraction (Takara Bio) of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled cRNA were prepared from 500 ng total RNA according to the standard Agilent protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 1.65 ug of cRNA were hybridized on a Whole Rat Genome Microarray 4x44K (Agilent) and wawhed according to the standard Agilent protocol.
|
| Scan protocol |
Microarrays were scanned using a DNA Microarray Scanner (Agilent).
|
| Description |
gene expression data from liver of rat fed a high-fat diet and high-fructose water without Eucommia ulmoides leaves
|
| Data processing |
The scanned images were analyzed with Agilent Feature Extraction 10.1.1.1 (Agilent Technologies) to obtain background subtracted and spatially detrended processed signal intensities.
|
| |
|
| Submission date |
Sep 27, 2010 |
| Last update date |
Aug 23, 2011 |
| Contact name |
Yukihiro Kobayashi |
| E-mail(s) |
yukihiro@kaiyodai.ac.jp
|
| Organization name |
Tokyo University of Marine Science and Technology
|
| Department |
Department of Marine Biosciences
|
| Lab |
Laboratory of Aquatic Resource Utilization
|
| Street address |
4-5-7, Konan
|
| City |
Minato-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
108-8477 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4135 |
| Series (1) |
| GSE24377 |
Gene expression data from liver of rat fed a high-fat diet and high-fructose water without/with Eucommia ulmoides leaves |
|
