|
| Status |
Public on Nov 13, 2010 |
| Title |
WT mouse 1h IL-6 stimulated rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Isolated hepatocytes from wildtype mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gp130 genotype: wild type
tissue: hepatocytes
duration of il-6 treatment: 1 h
|
| Treatment protocol |
Cells were starved overnight and stimulated with 200 ng/mL IL-6 for 0-12 hours under serum-free conditions.
|
| Growth protocol |
Hepatocytes from gp130– and gp130flox mice were isolated as described in J Exp Med. 2007 Aug;204(8):1935–1944.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TriFast-Reagent (peqLAB) and quality was controlled with an Experion Automated Electrophoresis System and an Implen NanoPhotometer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with Illumina TotalPrep RNA Amplification Kit from Applied Biosystems.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 5
|
| Data processing |
The data were normalised using cubic spline normalization with Illumina GenomeStudio version 1.6.0.
|
| |
|
| Submission date |
Nov 10, 2010 |
| Last update date |
Nov 12, 2010 |
| Contact name |
Frank Ruehle |
| E-mail(s) |
frank.ruehle@lifa-muenster.de
|
| Organization name |
Leibniz - Institut für Arterioskleroseforschung
|
| Street address |
Domagkstr. 3
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE25278 |
Gp130-dependent release of acute phase proteins is linked to the activation of innate immune signaling pathways |
|
