|
| Status |
Public on May 01, 2012 |
| Title |
hippocampus aged sedentary no. 20 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampus from aged sedentary
|
| Organism |
Mus musculus |
| Characteristics |
gender: Male
strain: BALB/c
age: 18-months-old
exercise condition: Sedentary
tissue: hippocampus
|
| Treatment protocol |
Mice were individually housing with or without a running wheel for 8 weeks.
|
| Growth protocol |
Mice were weaned at the age of 4 weeks, and afterwards supplied with standard nutrition until sacrification.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Animals were sacrificed by rapid decapitation without anesthesia. Hippocampal samples were dissected on a chilled glass dish and immediately placed into RNAlater solution (Qiagen, Valencia, CA) and stored at -20ºC until RNA isolation. Hippocampal samples were homogenized and RNA purified with the RNeasy Mini kit (Qiagen, Valencia, CA), then quantified and assessed for purity using a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE) and an Agilent 2100 bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the AMIL1791 Illumina® TotalPrep™ RNA Amplification Kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
May 04, 2011 |
| Last update date |
May 01, 2012 |
| Contact name |
Rachel Ann Kohman |
| Organization name |
University of Illinois
|
| Department |
Psychology
|
| Street address |
405 N Mathew
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE29075 |
Analysis of hippocampal gene expression in adult and aged male BABL/c mice with or without running wheel access |
|
