|
| Status |
Public on Jun 07, 2011 |
| Title |
HNG2 |
| Sample type |
RNA |
| |
|
| Source name |
HNG2
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Hepatic progenitor cells
strain: STOCK Tg(Nanog-GFP, Puro)1 Yam
development stage: E13.5
129/Sv x C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the ISOGEN kit (Nippon gene co, Tokyo Japan) following the manufacturer's recommendations. All RNA wwere quantified using a NanoDrop-1000 spectrophotometer.
|
| Label |
Cy3
|
| Label protocol |
Agilent one-color Low RNA Fluorecent Liner Amplification kit labeling protocol. Cyanine-3 (Cy3) labeled cRNA was prepared from 0.25 ug RNA according to the manufacturer's instructions. Dye incorporation and cRNA yield were checked with the NanoDrop Spectrophotometer.
|
| |
|
| Hybridization protocol |
Agilent one-color gene expression hyb/wash protocol. 1.65 ug of Cy3 labeled RNA was hybridized to Agilent Whole Mouse Genome microarrays (4 x 44K: GPL4134) at 60C for 17 hours and subsequently washed according to the Agilent standard hybridization protocol.
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2539A Microarray Scanner at 5 micron resolution. Images were quantified using Agilent Feature Extraction software version 9.5.3.1
|
| Data processing |
Agilent Feature Extraction software; gProcessedSignal; processed Cy3 Signal
|
| |
|
| Submission date |
Jun 03, 2011 |
| Last update date |
Jun 09, 2011 |
| Contact name |
Takafusa Hikichi |
| E-mail(s) |
thikichi@ri.ncgm.go.jp
|
| Organization name |
National Center for Global Health and Medicine
|
| Street address |
1-21-1 Toyama, Shinjuku-ku
|
| City |
Tokyo |
| ZIP/Postal code |
162-8655 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4134 |
| Series (2) |
| GSE29730 |
Unsupervised hierarchical clustering of iHPCs induced by 9 or 10 TFs |
| GSE29875 |
Systematic identification of transcription factors capable of inducing cell-type-specific transcriptional profiles |
|
