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Status |
Public on Oct 15, 2012 |
Title |
PE-0064-del |
Sample type |
genomic |
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|
Source name |
maternal leukocytes, delivery
|
Organism |
Homo sapiens |
Characteristics |
cell type: maternal leukocytes pregnancy status: delivery
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted using the QIAgen Flexigene Reagent kit and AutoGenFlex DNA purification kit from the frozen buffy coats, which were collected in a 10 ml EDTA tube, quantified with a NanoDrop spectrophotometer, normalized with standard PicoGreen methodology, and plated in 1000 ng aliquots.
|
Label |
Cy3 and Cy5
|
Label protocol |
Bisulfite modification was performed using the EZ DNA Methylation Kit (Zymo Research, Orange, CA).
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|
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Hybridization protocol |
Bisulphite-converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchips using the standard Illumina protocol.
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Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner settings.
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Description |
Bisulfite-modified DNA. VG0064P002
ArrayID: 1009701104
|
Data processing |
Illumina GenomeStudio (version 2010.2) was used to process the raw data. Average Beta and detection p-values from GenomeStudio are without normalization. Technical replicates were averaged. CpGs detected in less than 95% of samples were excluded.
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|
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Submission date |
May 02, 2012 |
Last update date |
Oct 15, 2012 |
Contact name |
Zhifu Sun |
Organization name |
Mayo Clinic
|
Department |
HSR-BSI
|
Street address |
200 1st ST SW
|
City |
Rochester |
State/province |
MN |
ZIP/Postal code |
55905 |
Country |
USA |
|
|
Platform ID |
GPL8490 |
Series (1) |
GSE37722 |
Normal early pregnancy: a transient state of epigenetic change favoring hypomethylation |
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