|
| Status |
Public on Oct 15, 2012 |
| Title |
PE-0069-del |
| Sample type |
genomic |
| |
|
| Source name |
maternal leukocytes, delivery
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: maternal leukocytes
pregnancy status: delivery
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using the QIAgen Flexigene Reagent kit and AutoGenFlex DNA purification kit from the frozen buffy coats, which were collected in a 10 ml EDTA tube, quantified with a NanoDrop spectrophotometer, normalized with standard PicoGreen methodology, and plated in 1000 ng aliquots.
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Bisulfite modification was performed using the EZ DNA Methylation Kit (Zymo Research, Orange, CA).
|
| |
|
| Hybridization protocol |
Bisulphite-converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchips using the standard Illumina protocol.
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner settings.
|
| Description |
Bisulfite-modified DNA.
VG0069P002
ArrayID: 1009701107
|
| Data processing |
Illumina GenomeStudio (version 2010.2) was used to process the raw data. Average Beta and detection p-values from GenomeStudio are without normalization. Technical replicates were averaged. CpGs detected in less than 95% of samples were excluded.
|
| |
|
| Submission date |
May 02, 2012 |
| Last update date |
Oct 15, 2012 |
| Contact name |
Zhifu Sun |
| Organization name |
Mayo Clinic
|
| Department |
HSR-BSI
|
| Street address |
200 1st ST SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL8490 |
| Series (1) |
| GSE37722 |
Normal early pregnancy: a transient state of epigenetic change favoring hypomethylation |
|
